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Literature summary extracted from
- Purification and characterization of the monooxygenase catalyzing sulfur-atom specific oxidation of dibenzothiophene and benzothiophene from the thermophilic bacterium Paenibacillus sp. strain A11-2 (2003), Appl. Microbiol. Biotechnol., 60, 128-133.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | 1,10-phenanthroline | complete inhibition at 1 mM | Paenibacillus sp. |  |
| 1.14.14.21 | 5,5'-dithio-bis-2-nitrobenzoic acid | 94% inhibition at 0.5 mM | Paenibacillus sp. | |
| 1.14.14.21 | CaCl2 | 16% inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | CoCl2 | 74% inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | CuCl2 | complete inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | EDTA | 68% inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | iodoacetate | 46% inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | MnCl2 | 66% inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | N-ethylmaleimide | 98% inhibition at 1 mM | Paenibacillus sp. | |
| 1.14.14.21 | p-chloromercuribenzoic acid | complete inhibition at 0.5 mM | Paenibacillus sp. | |
| 1.14.14.21 | ZnCl2 | complete inhibition at 1 mM | Paenibacillus sp. | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.21 | 0.36 | - |
benzothiophene | pH 7.0, 55°C | Paenibacillus sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 43000 | - |
4 * 43000, SDS-PAGE | Paenibacillus sp. |
| 1.14.14.21 | 200000 | - |
gel filtration | Paenibacillus sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.21 | Paenibacillus sp. | Q9LBX2 | - |
- |
| 1.14.14.21 | Paenibacillus sp. A11-2 | Q9LBX2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.14.21 | native enzyme 116fold from strain All-2 by two different steps of anion exchange chromatography and dialysis, followed by hydrophobic interaction and hydroxyapatite chromatography | Paenibacillus sp. |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 18.6 | - |
purified enzyme, pH 7.0, 55°C | Paenibacillus sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.21 | 2-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 2-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3-methylbenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 3-methylbenzothiophene-1,1-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 3-methyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 3-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-diethyldibenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 4,6-diethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. A11-2 | 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 4-methyldibenzothiophene + 2 FMNH2 + 2 O2 | low activity | Paenibacillus sp. A11-2 | 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | 5-methylbenzothiophene + 2 FMNH2 + 2 O2 | - |
Paenibacillus sp. | 5-methylbenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | benzothiophene + 2 FMNH2 + 2 O2 | best substrate | Paenibacillus sp. | benzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | benzothiophene + 2 FMNH2 + 2 O2 | best substrate | Paenibacillus sp. A11-2 | benzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | low reactivity of TdsC toward dibenzothiophene at high substrate concentrations | Paenibacillus sp. | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | dibenzothiophene + 2 FMNH2 + 2 O2 | low reactivity of TdsC toward dibenzothiophene at high substrate concentrations | Paenibacillus sp. A11-2 | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
| 1.14.14.21 | additional information | substrate specificity of enzyme TsdC with dibenzothiophene derivatives and benzothiophene derivatives, overview. No or poor activity with 4,6-dipropyldibenzothiophene, 4,6-dibutyldibenzothiophene, and 1-methyldibenzothiophene | Paenibacillus sp. | ? | - |
? | |
| 1.14.14.21 | additional information | substrate specificity of enzyme TsdC with dibenzothiophene derivatives and benzothiophene derivatives, overview. No or poor activity with 4,6-dipropyldibenzothiophene, 4,6-dibutyldibenzothiophene, and 1-methyldibenzothiophene | Paenibacillus sp. A11-2 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | homotetramer | 4 * 43000, SDS-PAGE | Paenibacillus sp. |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.21 | benzothiophene monooxygenase | - |
Paenibacillus sp. |
| 1.14.14.21 | BT monooxygenase | - |
Paenibacillus sp. |
| 1.14.14.21 | DBT monooxygenase | - |
Paenibacillus sp. |
| 1.14.14.21 | dibenzothiophene monooxygenase | - |
Paenibacillus sp. |
| 1.14.14.21 | TdsC | - |
Paenibacillus sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 65 | - |
- |
Paenibacillus sp. |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 30 | 65 | activity range | Paenibacillus sp. |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 65 | - |
purified enzyme, retains more than 80% of its activity after exposure for 30 min | Paenibacillus sp. |
| 1.14.14.21 | 70 | - |
purified enzyme, no activity remaining after 30 min | Paenibacillus sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.21 | 9 | - |
- |
Paenibacillus sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.21 | FMNH2 | - |
Paenibacillus sp. | |
| 1.14.14.21 | additional information | FAD and lumiflavin have only weak activity as reducing agents | Paenibacillus sp. | |
| 1.14.14.21 | additional information | FMN oxidoreductase TdsD, a NADH-dependent FMN oxidoreductase, absolutely required for TdsC activity, maximum activity is obtained at a TdsD/TdsC molar ratio of 0.5, no activity in the absence of TdsD | Paenibacillus sp. | |
| 1.14.14.21 | NADH | - |
Paenibacillus sp. | |
| 1.14.14.21 | NADPH | can substitute for NADH to some extent | Paenibacillus sp. | |
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