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Literature summary extracted from

  • Takenaka, S.; Yoshida, K.; Tanaka, K.; Yoshida, K.
    Molecular characterization of a novel N-acetyltransferase from Chryseobacterium sp. (2014), Appl. Environ. Microbiol., 80, 1770-1776.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.B37 expressed in Escherichia coli BL21(DE3) cells Chryseobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.B37 4-chloromercuribenzoate 35.2% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 AgNO3 22.4% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 Cu2+ 79.5% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 diethyldicarbonate 49.3% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 diethylpyrocarbonate 35.2% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 Hg2+ 19.8% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 Mg2+ 94.2% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 additional information Mn2+, EDTA, tiron, alpha,alpha'-dipyridyl, phenylglyoxal, phenylmethylsulfonyl fluoride, iodoacetic acid and iodoacetamide do not markedly inhibit the enzyme Chryseobacterium sp.
2.3.1.B37 N-ethylmaleimide 34.5% residual activity after 1 h at 1 mM Chryseobacterium sp.
2.3.1.B37 o-phenanthroline 93.1% residual activity after 1 h at 1 mM Chryseobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B37 0.145
-
 L-2-phenylglycine at pH 7.5 and 30°C Chryseobacterium sp.
2.3.1.B37 0.77
-
 L-2-phenylglycine methyl ester at pH 7.5 and 30°C Chryseobacterium sp.
2.3.1.B37 1.41
-
 L-2-chlorophenylglycine at pH 7.5 and 30°C Chryseobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.B37 18000
-
 x * 18000, SDS-PAGE Chryseobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B37 Chryseobacterium sp. W0SLW8
-
-
2.3.1.B37 Chryseobacterium sp. 5-3B W0SLW8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.B37 ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, and Cosmogel His-accept column chromatography Chryseobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.B37 0.55
-
 cell extract, at pH 7.5 and 30°C Chryseobacterium sp.
2.3.1.B37 10.5
-
 purified enzyme, at pH 7.5 and 30°C Chryseobacterium sp.

Storage Stability

EC Number Storage Stability Organism
2.3.1.B37 4°C, 50 mM sodium potassium phosphate buffer (pH 7.5) containing 0.3 M NaCl, more than 1 month, no loss of activity Chryseobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B37 acetyl-CoA + (+/-)-1-phenylethylamine 6.3% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + (+/-)-1-phenylethylamine 6.3% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + DL-2-chlorophenylglycine 56.9% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + DL-2-chlorophenylglycine 56.9% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + DL-2-phenylglycine 98% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + DL-2-phenylglycine 98% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + DL-4-chlorophenylglycine 7.4% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + DL-4-chlorophenylglycine 7.4% activity compared to L-2-phenylglycine Chryseobacterium sp. 5-3B CoA + ?
-
 ?
2.3.1.B37 acetyl-CoA + L-2-chlorophenylglycine 52.9% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + N-acetyl-L-2-chlorophenylglycine
-
 ?
2.3.1.B37 acetyl-CoA + L-2-phenylglycine 100% activity Chryseobacterium sp. CoA + (2S)-2-acetylamino-2-phenylacetic acid
-
 ?
2.3.1.B37 acetyl-CoA + L-2-phenylglycine methyl ester 75.5% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + N-acetyl-L-2-phenylglycine methyl ester
-
 ?
2.3.1.B37 acetyl-CoA + L-4-hydroxyphenylglycine 16.7% activity compared to L-2-phenylglycine Chryseobacterium sp. CoA + N-acetyl-L-4-hydroxyphenylglycine
-
 ?
2.3.1.B37 additional information the enzyme shows low activity for 5-aminosalicylic acid (5.5%), 4-aminobenzoic acid (2.5%), D-4-hydroxyphenylglycine (2.4%), DL-homophenylalanine (1.9%), DL-phenylalanine (1.5%), and 5-hydroxytryptamine and D-2-phenylglycine (less than 1%, respectively) Chryseobacterium sp. ?
-
 ?
2.3.1.B37 additional information the enzyme shows low activity for 5-aminosalicylic acid (5.5%), 4-aminobenzoic acid (2.5%), D-4-hydroxyphenylglycine (2.4%), DL-homophenylalanine (1.9%), DL-phenylalanine (1.5%), and 5-hydroxytryptamine and D-2-phenylglycine (less than 1%, respectively) Chryseobacterium sp. 5-3B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.B37 ? x * 18000, SDS-PAGE Chryseobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B37 NatA
-
 Chryseobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.B37 60
-
-
 Chryseobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.B37 7.31
-
 L-2-chlorophenylglycine at pH 7.5 and 30°C Chryseobacterium sp.
2.3.1.B37 13.6
-
 L-2-phenylglycine at pH 7.5 and 30°C Chryseobacterium sp.
2.3.1.B37 19.2
-
 L-2-phenylglycine methyl ester at pH 7.5 and 30°C Chryseobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.B37 7.5
-
-
 Chryseobacterium sp.