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Literature summary extracted from

  • Galimand, M.; Fishovitz, J.; Lambert, T.; Barbe, V.; Zajicek, J.; Mobashery, S.; Courvalin, P.
    AAC(3)-XI, a new aminoglycoside 3-N-acetyltransferase from Corynebacterium striatum (2015), Antimicrob. Agents Chemother., 59, 5647-5653.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.81 gne aac(3)-XI, DNA and amino acid sequence determination and analysis, genomic organization, the aac(3)-XI gene is located downstream from a gene for a transposase (TnpCx) and upstream from that for a two-component system sensor kinase, the sequence upstream from aac(3)-XI does not reveal any putative promoter, recombinant expression of His-tagged enzyme in Escherichia coli strains TOP10 and BL21 Star(DE3) Corynebacterium striatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.81 additional information strain BM4687 is resistant to gentamicin and tobramycin but susceptible to kanamycin A and amikacin Corynebacterium striatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.81 additional information
-
 additional information Michaelis-Menten kinetics Corynebacterium striatum
2.3.1.81 0.0027
-
 acetyl-CoA pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.0027
-
 acetyl-CoA recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.0092
-
 dibekacin pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.0092
-
 dibekacin recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.0138
-
 kanamycin B pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.0138
-
 kanamycin B recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.81 acetyl-CoA + a 2-deoxystreptamine antibiotic Corynebacterium striatum
-
 CoA + N3-acetyl-2-deoxystreptamine antibiotic
-
 ?
2.3.1.81 acetyl-CoA + a 2-deoxystreptamine antibiotic Corynebacterium striatum BM4687
-
 CoA + N3-acetyl-2-deoxystreptamine antibiotic
-
 ?
2.3.1.81 acetyl-CoA + dibekacin Corynebacterium striatum
-
 CoA + N3-acetyldibekacin
-
 ?
2.3.1.81 acetyl-CoA + dibekacin Corynebacterium striatum BM4687
-
 CoA + N3-acetyldibekacin
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B Corynebacterium striatum
-
 CoA + N3-acetylkanamycin B
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B Corynebacterium striatum BM4687
-
 CoA + N3-acetylkanamycin B
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.81 Corynebacterium striatum
-
 strain isolated from the heel biopsy specimen of an 80-year-old diabetic woman
-
2.3.1.81 Corynebacterium striatum A0A0K2X0F2 gene aac(3)-XI
-
2.3.1.81 Corynebacterium striatum BM4687
-
 strain isolated from the heel biopsy specimen of an 80-year-old diabetic woman
-
2.3.1.81 Corynebacterium striatum BM4687 A0A0K2X0F2 gene aac(3)-XI
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.81 recombinant His-tagged enzyme from Escherichia coli strain BL21 Star(DE3) by nickel affinity chromatography and ultrafiltration Corynebacterium striatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.81 acetyl-CoA + a 2-deoxystreptamine antibiotic
-
 Corynebacterium striatum CoA + N3-acetyl-2-deoxystreptamine antibiotic
-
 ?
2.3.1.81 acetyl-CoA + a 2-deoxystreptamine antibiotic
-
 Corynebacterium striatum BM4687 CoA + N3-acetyl-2-deoxystreptamine antibiotic
-
 ?
2.3.1.81 acetyl-CoA + dibekacin
-
 Corynebacterium striatum CoA + N3''-acetyldibekacin
-
 ?
2.3.1.81 acetyl-CoA + dibekacin
-
 Corynebacterium striatum BM4687 CoA + N3''-acetyldibekacin
-
 ?
2.3.1.81 acetyl-CoA + dibekacin
-
 Corynebacterium striatum CoA + N3-acetyldibekacin
-
 ?
2.3.1.81 acetyl-CoA + dibekacin
-
 Corynebacterium striatum CoA + N3-acetyldibekacin product identification by high-field NMR ?
2.3.1.81 acetyl-CoA + dibekacin
-
 Corynebacterium striatum BM4687 CoA + N3-acetyldibekacin
-
 ?
2.3.1.81 acetyl-CoA + dibekacin
-
 Corynebacterium striatum BM4687 CoA + N3-acetyldibekacin product identification by high-field NMR ?
2.3.1.81 acetyl-CoA + fortimicin
-
 Corynebacterium striatum CoA + N3-acetylfortimicin
-
 ?
2.3.1.81 acetyl-CoA + gentamicin C1A
-
 Corynebacterium striatum CoA + N3-acetylgentamicin C1A
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B
-
 Corynebacterium striatum CoA + N3'-acetylkanamycin B
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B
-
 Corynebacterium striatum BM4687 CoA + N3'-acetylkanamycin B
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B
-
 Corynebacterium striatum CoA + N3-acetylkanamycin B
-
 ?
2.3.1.81 acetyl-CoA + kanamycin B
-
 Corynebacterium striatum BM4687 CoA + N3-acetylkanamycin B
-
 ?
2.3.1.81 acetyl-CoA + sisomicin
-
 Corynebacterium striatum CoA + N3-acetylsisomicin
-
 ?
2.3.1.81 acetyl-CoA + tobramycin
-
 Corynebacterium striatum CoA + N3-acetyltobramycin
-
 ?
2.3.1.81 additional information the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin Corynebacterium striatum ?
-
 ?
2.3.1.81 additional information the enzyme shows a unique profile of the reaction. No activity with kanamycin A, amikacin, netilmicin, neomycin B, and apramycin Corynebacterium striatum BM4687 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.81 AAC(3)-XI
-
 Corynebacterium striatum
2.3.1.81 aminoglycoside 3-N-acetyltransferase type XI
-
 Corynebacterium striatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.81 37
-
 assay at Corynebacterium striatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.81 0.17
-
 dibekacin pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.17
-
 dibekacin recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.23
-
 kanamycin B pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.23
-
 kanamycin B recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.24
-
 acetyl-CoA pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 0.24
-
 acetyl-CoA recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.81 6
-
 assay at Corynebacterium striatum

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.81 acetyl-CoA
-
 Corynebacterium striatum

General Information

EC Number General Information Comment Organism
2.3.1.81 evolution aminoglycoside 3-N-acetyltransferases [AAC(3)] are widely distributed in Gram-negative bacteria and are categorized in five types (I, II, III, IV, and VI). To date, only four are identified in the Gram-positive aminoglycoside producers, overview Corynebacterium striatum
2.3.1.81 physiological function expression in Escherichia coli confers resistance to gentamicin, tobramycin, sisomicin, dibekacin, and fortimicin, the MICs of kanamycin A, netilmicin, amikacin, apramycin, and neomycin B remaining unchanged Corynebacterium striatum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.81 17
-
 kanamycin B pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 17
-
 kanamycin B recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 18
-
 dibekacin pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 18
-
 dibekacin recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 89
-
 acetyl-CoA pH 6.0, 37°C Corynebacterium striatum
2.3.1.81 89
-
 acetyl-CoA recombinant His-tagged enzyme, pH 6.0, 37°C Corynebacterium striatum