Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.; Bhuiya, M.; Zhou, R.; Yu, O.
    Pterostilbene production by microorganisms expressing resveratrol O-methyltransferase (2015), Ann. Microbiol., 65, 817-826.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.240 synthesis the enzyme is useful for conversion of 4-coumaric acid to pterostilbene via co-expression of 4CL::STS and ROMT in recombinant Escherichia coli and Saccharomyces cerevisiae, overview Vitis vinifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.240 gene ROMT, recombinant expression in Escherichia coli strain BW27784 and in Saccharomyces cerevisiae, coexpression of two additional genes from the resveratrol biosynthetic pathway, 4-coumarate CoA-ligase and stilbene synthase, leads to production of a large amount of pterostilbene with a trace amount of pinostilbene, subcloning in Escherichia coli strain DH5alpha, recombinant expression of grape SUMO-fusion wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Vitis vinifera

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.240 D174A site-directed mutagenesis, almost inactive mutant Vitis vinifera
2.1.1.240 F167A site-directed mutagenesis, inactive mutant Vitis vinifera
2.1.1.240 H261A site-directed mutagenesis, inactive mutant Vitis vinifera
2.1.1.240 W258A site-directed mutagenesis, inactive mutant Vitis vinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.240 52000
-
 x * 52000, recombinant SUMO-ROMT enzyme, SDS-PAGE Vitis vinifera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene Vitis vinifera i.e. pinostilbene S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol Vitis vinifera
-
 S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.240 Vitis vinifera B6VJS4 gene ROMT
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene Vitis vinifera S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol
-
 Vitis vinifera S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.240 ? x * 52000, recombinant SUMO-ROMT enzyme, SDS-PAGE Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
2.1.1.240 resveratrol O-methyltransferase
-
 Vitis vinifera

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.240 S-adenosyl-L-methionine
-
 Vitis vinifera

General Information

EC Number General Information Comment Organism
2.1.1.240 additional information three-dimensional enzyme homology modeling and docking study for identification of four key catalytic residues. Residues F167 and W258 form a sandwich to bind resveratrol, residue D174 is in close proximity to the substrate, and residue H261 might serve as a general base in the deprotonation of hydroxyl groups Vitis vinifera