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Literature summary extracted from

  • Abdoul-Zabar, J.; Sorel, I.; Helaine, V.; Charmantray, F.; Devamani, T.; Yi, D.; De Berardinis, V.; Louis, D.; Marliere, P.; Fessner, W.; Hecquet, L.
    Thermostable transketolase from Geobacillus stearothermophilus: Characterization and catalytic properties (2013), Adv. Synth. Catal., 355, 116-128.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1 expressed in Escherichia coli BL21(DE3) cells Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.13
-
 D-ribose-5-phosphate at pH 7.5 and 50°C Geobacillus stearothermophilus
2.2.1.1 0.2
-
 D-ribose-5-phosphate at pH 7.5 and 25°C Geobacillus stearothermophilus
2.2.1.1 5.2
-
 L-erythrulose at pH 7.5 and 25°C Geobacillus stearothermophilus
2.2.1.1 8
-
 L-erythrulose at pH 7.5 and 50°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+ required Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 D-xylulose-5-phosphate + D-ribose-5-phosphate Geobacillus stearothermophilus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Geobacillus stearothermophilus A0A0I9QGZ2 formerly Bacillus stearothermophilus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.1 Ni-NTA column chromatography Geobacillus stearothermophilus

Storage Stability

EC Number Storage Stability Organism
2.2.1.1 50°C, 7 days, no loss of activity Geobacillus stearothermophilus
2.2.1.1 65°C, 3 days, no loss of activity Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 butanal + hydroxypyruvate
-
 Geobacillus stearothermophilus 1,3-dihydroxyhexan-2-one + CO2
-
 ?
2.2.1.1 butyraldehyde + hydroxypyruvate
-
 Geobacillus stearothermophilus ? + CO2
-
 ?
2.2.1.1 D-glyceraldehyde + hydroxypyruvate
-
 Geobacillus stearothermophilus D-xylulose + CO2
-
 ?
2.2.1.1 D-ribose + hydroxypyruvate
-
 Geobacillus stearothermophilus ? + CO2
-
 ?
2.2.1.1 D-xylulose-5-phosphate + D-ribose-5-phosphate
-
 Geobacillus stearothermophilus ?
-
 ?
2.2.1.1 DL-glyceraldehyde + hydroxypyruvate
-
 Geobacillus stearothermophilus ? + CO2
-
 ?
2.2.1.1 glycolaldehyde + hydroxypyruvate
-
 Geobacillus stearothermophilus L-erythrulose + CO2
-
 ?
2.2.1.1 L-erythrulose + D-ribose-5-phosphate
-
 Geobacillus stearothermophilus D-sedoheptulose 7-phosphate + glycolaldehyde
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.1 60 70
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.1 40 70 more than 50% activity from 40 to 70°C with a sharp decrease thereafter leaving about 25% and 0% activity at 75°C and 85°C, respectively Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.1 50 85 the enzyme shows a half-life of 35 min at 50°C, 15 min at 75°C, and is immediately inactivated at 85°C Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.1 7 8
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.2.1.1 5.5 8.5 more than 50% activity between pH 5.5 and 8.5 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Geobacillus stearothermophilus