EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.9 | recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cupriavidus necator |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.9 | purified recombinant enzyme, sitting drop vapour diffusion method, mixing of 0.001 ml of 140 mg/ml protein in 40 mM Tris-HCl, pH 8.0, with 0.001 ml of reservoir solution containing 17% PEG 8000, 0.1 M HEPES pH 7.0, and equilibration against 0.5 ml of reservoir solution, 20°C, 7 days, X-ray diffraction structure determination and analysis at 1.4 A resolution | Cupriavidus necator |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 0.158 | - |
acetoacetyl-CoA | pH 8.3, 30°C, recombinant enzyme | Cupriavidus necator |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.9 | Mg2+ | required | Cupriavidus necator |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 40000 | - |
2 * 40000, recombinant His-tagged enzyme, SDS-PAGE | Cupriavidus necator |
2.3.1.9 | 80000 | - |
recombinant His-tagged enzyme, gel filtration | Cupriavidus necator |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.9 | 2 acetyl-CoA | Cupriavidus necator | - |
CoA + acetoacetyl-CoA | - |
r | |
2.3.1.9 | 2 acetyl-CoA | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
CoA + acetoacetyl-CoA | - |
r | |
2.3.1.9 | CoA + acetoacetyl-CoA | Cupriavidus necator | - |
2 acetyl-CoA | - |
r | |
2.3.1.9 | CoA + acetoacetyl-CoA | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | - |
2 acetyl-CoA | - |
r | |
2.3.1.9 | additional information | Cupriavidus necator | the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA | ? | - |
? | |
2.3.1.9 | additional information | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.9 | Cupriavidus necator | Q0KAI3 | gene H16_A1887; gene H16_A1887 | - |
2.3.1.9 | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | Q0KAI3 | gene H16_A1887; gene H16_A1887 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.9 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration | Cupriavidus necator |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.9 | 2 acetyl-CoA | - |
Cupriavidus necator | CoA + acetoacetyl-CoA | - |
r | |
2.3.1.9 | 2 acetyl-CoA | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | CoA + acetoacetyl-CoA | - |
r | |
2.3.1.9 | CoA + acetoacetyl-CoA | - |
Cupriavidus necator | 2 acetyl-CoA | - |
r | |
2.3.1.9 | CoA + acetoacetyl-CoA | - |
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | 2 acetyl-CoA | - |
r | |
2.3.1.9 | additional information | the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA | Cupriavidus necator | ? | - |
? | |
2.3.1.9 | additional information | the enzyme shows degradative thiolase activity by converting 3-ketoacyl-CoA to acyl-CoA | Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.9 | homodimer | 2 * 40000, recombinant His-tagged enzyme, SDS-PAGE | Cupriavidus necator |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.9 | 3-ketoacyl-CoA thiolase | - |
Cupriavidus necator |
2.3.1.9 | A1887 | - |
Cupriavidus necator |
2.3.1.9 | ACAT | - |
Cupriavidus necator |
2.3.1.9 | acetoacetyl-CoA thiolase | - |
Cupriavidus necator |
2.3.1.9 | KACT | - |
Cupriavidus necator |
2.3.1.9 | type II thiolase | - |
Cupriavidus necator |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 30 | - |
assay at | Cupriavidus necator |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 5000000 | - |
acetoacetyl-CoA | pH 8.3, 30°C, recombinant enzyme | Cupriavidus necator |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.9 | 8.3 | - |
assay at | Cupriavidus necator |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.9 | metabolism | the enzyme shows degradative thiolase activity catalyzing the fourth step of beta-oxidation degradative pathways by converting 3-ketoacyl-CoA to acyl-CoA | Cupriavidus necator |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.9 | 31650 | - |
acetoacetyl-CoA | pH 8.3, 30°C, recombinant enzyme | Cupriavidus necator |