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Literature summary extracted from

  • Fan, S.; Li, D.; Fleming, J.; Hong, Y.; Chen, T.; Zhou, L.; Bi, L.; Wang, D.; Zhang, X.; Chen, G.
    Purification and X-ray crystallographic analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from Mycobacterium smegmatis (2014), Acta Crystallogr. Sect. F, 70, 1372-1375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.47 gene bioF, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3) Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.47 purified recombinant enzyme, hanging-drop vapour diffusion, mixing of 0.002 ml of 5 mg/ml protein in 20 mM Tris-HCl pH 7.9, 100 mM NaCl, 2 mM DTT, with 0.002 ml of reservoir solution containing 150 mM DL-malic acid pH 7.4, 100 mM NaCl, 25% PEG 3350, equilibrqtion against 0.2 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.47 42000
-
 x * 42000, recombinant His-tagged enzyme, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine Mycolicibacterium smegmatis
-
 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine Mycolicibacterium smegmatis ATCC 700084
-
 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.47 Mycolicibacterium smegmatis A0QX65 gene bioF
-
2.3.1.47 Mycolicibacterium smegmatis ATCC 700084 A0QX65 gene bioF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.47 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography, desalting gel filtration, and anion exchange chromatography followed by gel filtration, to over 95% purity Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine
-
 Mycolicibacterium smegmatis 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine
-
 Mycolicibacterium smegmatis ATCC 700084 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.47 ? x * 42000, recombinant His-tagged enzyme, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.47 7-keto-8-aminopelargonic acid synthase
-
 Mycolicibacterium smegmatis
2.3.1.47 BioF
-
 Mycolicibacterium smegmatis
2.3.1.47 KAPA synthase
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.47 pyridoxal 5'-phosphate dependent on Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
2.3.1.47 metabolism the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid Mycolicibacterium smegmatis