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Literature summary extracted from

  • Liu, Y.; Zhang, Y.; Cao, X.; Xue, S.
    Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of MCAT from Synechocystis sp. PCC 6803 (2013), Acta Crystallogr. Sect. F, 69, 1256-1259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 gene fabD or slr2023, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, recombinant expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Synechocystis sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.39 purified recombinant enzyme, X-ray diffraction structure determination and analysis Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] Synechocystis sp.
-
 CoA + a malonyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Synechocystis sp. P73242 gene fabD or slr2023
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.39 recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 95% purity Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein]
-
 Synechocystis sp. CoA + a malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 malonyl-coenzymeA:acyl-carrier protein transacylase
-
 Synechocystis sp.
2.3.1.39 MCAT
-
 Synechocystis sp.