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Literature summary extracted from

  • Fang, W.; Robinson, D.A.; Raimi, O.G.; Blair, D.E.; Harrison, J.R.; Lockhart, D.E.; Torrie, L.S.; Ruda, G.F.; Wyatt, P.G.; Gilbert, I.H.; van Aalten, D.M.
    N-myristoyltransferase is a cell wall target in Aspergillus fumigatus (2015), ACS Chem. Biol., 10, 1425-1434.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 expression in Escherichia coli Aspergillus fumigatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.97 in complex with inhibitors 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide and 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide binding in the peptide substrate pocket Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide inhibits the enzyme and is fungicidal under partially repressive nmt conditions Aspergillus fumigatus
2.3.1.97 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
-
 Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 0.0008
-
 myristoleoyl-CoA pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus
2.3.1.97 0.022
-
 CAP5.5 pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Aspergillus fumigatus Q9UVX3
-
-
2.3.1.97 Aspergillus fumigatus ATCC MYA-4609 Q9UVX3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 myristoleoyl-CoA + CAP5.5 acceptor is biotin-tagged cytoskeleton-associated protein 5.5 Aspergillus fumigatus N-myristoleoyl-CAP5.5 + CoA
-
 ?
2.3.1.97 myristoleoyl-CoA + CAP5.5 acceptor is biotin-tagged cytoskeleton-associated protein 5.5 Aspergillus fumigatus ATCC MYA-4609 N-myristoleoyl-CAP5.5 + CoA
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.97 0.000012
-
 pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus 2,6-dichloro-4-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-N-(1,5-dimethyl-3-isobutyl-1H-pyrazol-4-yl)benzenesulfonamide
2.3.1.97 0.000023
-
 pH not specified in the publication, temperature not specified in the publication Aspergillus fumigatus 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide

General Information

EC Number General Information Comment Organism
2.3.1.97 physiological function expression of the Nmt gene is essential for viability. Partial repression of the gene reveals downstream effects of N-myristoylation on cell wall morphology Aspergillus fumigatus