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Literature summary extracted from

  • Mohan Kumar, N.S.; Manonmani, H.K.
    Purification, characterization and kinetic properties of extracellular L-asparaginase produced by Cladosporium sp (2013), World J. Microbiol. Biotechnol., 29, 577-587.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.1 food industry the enzyme reduces acrylamide content in starchy fried food commodities Cladosporium sp.

General Stability

EC Number General Stability Organism
3.5.1.1 in-vitro half-life of purified L-asparaginase in mammalian blood serum is 93.69 h Cladosporium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 2-mercaptoethanol
-
 Cladosporium sp.
3.5.1.1 Ca2+
-
 Cladosporium sp.
3.5.1.1 Co2+
-
 Cladosporium sp.
3.5.1.1 Cu2+
-
 Cladosporium sp.
3.5.1.1 K+
-
 Cladosporium sp.
3.5.1.1 Mg2+
-
 Cladosporium sp.
3.5.1.1 Na+
-
 Cladosporium sp.
3.5.1.1 Zn2+
-
 Cladosporium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 100
-
 L-asparagine at pH 6.3 and 30°C Cladosporium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Fe3+ stimulates activity Cladosporium sp.
3.5.1.1 KI stimulates activity Cladosporium sp.
3.5.1.1 Pb2+ stimulates activity Cladosporium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 37000
-
 2 * 37000 + 1 * 47000, SDS-PAGE Cladosporium sp.
3.5.1.1 47000
-
 2 * 37000 + 1 * 47000, SDS-PAGE Cladosporium sp.
3.5.1.1 120000
-
 gel filtration Cladosporium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Cladosporium sp. substrate hydrolysis efficiency of L-asparagine is 8fold higher than that of L-glutamine L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O Cladosporium sp.
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Cladosporium sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 culture condition:wheat bran-grown cell
-
 Cladosporium sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O substrate hydrolysis efficiency of L-asparagine is 8fold higher than that of L-glutamine Cladosporium sp. L-aspartate + NH3
-
 ?
3.5.1.1 L-glutamine + H2O
-
 Cladosporium sp. L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 heterotrimer 2 * 37000 + 1 * 47000, SDS-PAGE Cladosporium sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 L-asparaginase
-
 Cladosporium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 30
-
-
 Cladosporium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 6.3
-
-
 Cladosporium sp.