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Literature summary extracted from

  • Garg, A.; Lukk, T.; Kumar, V.; Choi, J.Y.; Augagneur, Y.; Voelker, D.R.; Nair, S.; Ben Mamoun, C.
    Structure, function and inhibition of the phosphoethanolamine methyltransferases of the human malaria parasites Plasmodium vivax and Plasmodium knowlesi (2015), Sci. Rep., 5, 9064.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 expressed in Escherichia coli BL21(DE3) cells Plasmodium knowlesi
2.1.1.103 expressed in Escherichia coli BL21(DE3) cells Plasmodium vivax

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.103 hanging drop vapor diffusion method, using 100 mM Na-acetate (pH 5.0), and 20% (w/v) PEG 3350 Plasmodium knowlesi
2.1.1.103 sitting drop vapor diffusion method, using 200 mM NH4-formate and 20% (w/v) PEG 3350 Plasmodium vivax

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.103 amodiaquine
-
 Plasmodium knowlesi
2.1.1.103 amodiaquine
-
 Plasmodium vivax
2.1.1.103 Chloroquine
-
 Plasmodium knowlesi
2.1.1.103 Chloroquine
-
 Plasmodium vivax
2.1.1.103 NSC158011
-
 Plasmodium knowlesi
2.1.1.103 NSC158011
-
 Plasmodium vivax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.1
-
 Ethanolamine phosphate at pH 8.6 and 37°C Plasmodium knowlesi
2.1.1.103 0.21
-
 Ethanolamine phosphate at pH 8.6 and 37°C Plasmodium vivax
2.1.1.103 0.23
-
 S-adenosyl-L-methionine at pH 8.6 and 37°C Plasmodium vivax
2.1.1.103 0.27
-
 S-adenosyl-L-methionine at pH 8.6 and 37°C Plasmodium knowlesi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.103 31000
-
 x * 31000, SDS-PAGE Plasmodium knowlesi
2.1.1.103 31000
-
 x * 31000, SDS-PAGE Plasmodium vivax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Plasmodium knowlesi
-
 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Plasmodium vivax
-
 S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Plasmodium knowlesi B3L8G9
-
-
2.1.1.103 Plasmodium vivax A5K867
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.103 nickel HisTrap column chromatography and Superdex 75PG gel filtration Plasmodium knowlesi
2.1.1.103 nickel HisTrap column chromatography and Superdex 75PG gel filtration Plasmodium vivax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Plasmodium knowlesi S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Plasmodium vivax S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.103 ? x * 31000, SDS-PAGE Plasmodium knowlesi
2.1.1.103 ? x * 31000, SDS-PAGE Plasmodium vivax

Synonyms

EC Number Synonyms Comment Organism
2.1.1.103 phosphoethanolamine methyltransferase
-
 Plasmodium knowlesi
2.1.1.103 phosphoethanolamine methyltransferase
-
 Plasmodium vivax
2.1.1.103 PMT
-
 Plasmodium knowlesi
2.1.1.103 PMT
-
 Plasmodium vivax

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.103 0.05
-
 at pH 8.6 and 37°C Plasmodium knowlesi NSC158011
2.1.1.103 0.099
-
 at pH 8.6 and 37°C Plasmodium vivax NSC158011
2.1.1.103 0.146
-
 at pH 8.6 and 37°C Plasmodium knowlesi Chloroquine
2.1.1.103 0.146
-
 at pH 8.6 and 37°C Plasmodium vivax Chloroquine
2.1.1.103 0.22
-
 at pH 8.6 and 37°C Plasmodium vivax amodiaquine
2.1.1.103 0.398
-
 at pH 8.6 and 37°C Plasmodium knowlesi amodiaquine