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Literature summary extracted from

  • Hering, S.; Sieg, A.; Kreikemeyer, B.; Fiedler, T.
    Kinetic characterization of arginine deiminase and carbamate kinase from Streptococcus pyogenes M49 (2013), Protein Expr. Purif., 91, 61-68.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.6 additional information no significant changes in enzyme activity are observed in the presence of 10 mM ATP, citrulline, and carbamoyl phosphate, respectively Streptococcus pyogenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.2 gene arcC, genetic organization in the arc operon, sequence comparisons, recombinant expression in Escherichia coli strain DH5alpha Streptococcus pyogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.2 ATP
-
 Streptococcus pyogenes
2.7.2.2 L-arginine slight inhibition Streptococcus pyogenes
2.7.2.2 L-citrulline
-
 Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.2 0.65
-
 Carbamoyl phosphate recombinant enzyme, pH 6.5, 37°C Streptococcus pyogenes
2.7.2.2 0.72
-
 ADP recombinant enzyme, pH 6.5, 37°C Streptococcus pyogenes
3.5.3.6 1.33
-
 L-arginine at pH 6.5 and 37°C Streptococcus pyogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.2 Mg2+ required Streptococcus pyogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.2 34200
-
 x * 34200, SDS-PAGE Streptococcus pyogenes
3.5.3.6 47200
-
 x * 47200, SDS-PAGE Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate Streptococcus pyogenes
-
 ATP + NH3 + CO2
-
 r
2.7.2.2 ATP + NH3 + CO2 Streptococcus pyogenes
-
 ADP + carbamoyl phosphate
-
 r
3.5.3.6 L-arginine + H2O Streptococcus pyogenes
-
 L-citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Streptococcus pyogenes A0A0H3BY05 serotype M49, strain 591, gene arcC
-
3.5.3.6 Streptococcus pyogenes B5XMC3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.2 recombinant enzyme from Escherichia coli strain DH5alpha by affinity chromatography on StrepTactin resin Streptococcus pyogenes
3.5.3.6 StrepTactin Sepharose column chromatography Streptococcus pyogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.6 1.5
-
 purified enzyme, at pH 6.5 and 37°C Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
 Streptococcus pyogenes ATP + NH3 + CO2
-
 r
2.7.2.2 ATP + NH3 + CO2
-
 Streptococcus pyogenes ADP + carbamoyl phosphate
-
 r
3.5.3.6 L-arginine + H2O
-
 Streptococcus pyogenes L-citrulline + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.2 ? x * 34200, SDS-PAGE Streptococcus pyogenes
3.5.3.6 ? x * 47200, SDS-PAGE Streptococcus pyogenes

Synonyms

EC Number Synonyms Comment Organism
2.7.2.2 ArcC
-
 Streptococcus pyogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.2 37
-
-
 Streptococcus pyogenes
3.5.3.6 37
-
-
 Streptococcus pyogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.2 6.5
-
-
 Streptococcus pyogenes
3.5.3.6 6.5
-
-
 Streptococcus pyogenes

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.2 ADP
-
 Streptococcus pyogenes
2.7.2.2 ATP
-
 Streptococcus pyogenes

General Information

EC Number General Information Comment Organism
2.7.2.2 metabolism the enzyme is part of the the arginine deiminase system, overview Streptococcus pyogenes