Literature summary extracted from
Odunuga, O.; Adekoya, O.; Sylte, I.
High-level expression of pseudolysin, the extracellular elastase of Pseudomonas aeruginosa, in Escherichia coli and its purification (2015), Protein Expr. Purif., 113, 79-84.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.24.26 |
industry |
pseudolysin is a biotechnologically important enzyme in the tanning industry |
Pseudomonas aeruginosa |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.24.26 |
gene lasB, recombinant expression in Escherichia coli and in Pichia pastoris and secretion of the enzyme, large-scale expression of His-tagged enzyme under the control of the T7 promoter in Escherichia coli strain BL21(DE3) in inclusion bodies |
Pseudomonas aeruginosa |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.4.24.26 |
extracellular |
- |
Pseudomonas aeruginosa |
- |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.24.26 |
Zn2+ |
a zinc metalloprotease |
Pseudomonas aeruginosa |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.24.26 |
34160 |
- |
x * 34160, sequence calculation |
Pseudomonas aeruginosa |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.24.26 |
Pseudomonas aeruginosa |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.24.26 |
solubilized recombinant His-tagged enzyme from Escherichia coli cells by metal affinity chromatography, or the enzyme is simultaneously further purified, refolded and buffer-exchanged on a preparative Superdex 200 column by a modified urea reverse-gradient gel filtration |
Pseudomonas aeruginosa |
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
3.4.24.26 |
solubilization of recombinant His-tagged enzyme from strain BL21(DE3) in inclusion bodies by 8 M urea |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.4.24.26 |
3.2 |
- |
purified recombinant enzyme from Escherichia coli expression, pH 8.0, 25°C, substrate N-succinyl-Ala-Ala-Ala-4-nitroanilide |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.24.26 |
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O |
- |
Pseudomonas aeruginosa |
N-succinyl-Ala-Ala-Ala + 4-nitroaniline |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.24.26 |
? |
x * 34160, sequence calculation |
Pseudomonas aeruginosa |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.24.26 |
elastase |
- |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.24.26 |
25 |
- |
assay at |
Pseudomonas aeruginosa |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.24.26 |
8 |
- |
assay at |
Pseudomonas aeruginosa |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.4.24.26 |
evolution |
pseudolysin belongs to the thermolysin-like family of metallopeptidases |
Pseudomonas aeruginosa |