Literature summary extracted from

Laursen, M.; Gregersen, J.L.; Yatime, L.; Nissen, P.; Fedosova, N.U.
Structures and characterization of digoxin- and bufalin-bound Na+,K+-ATPase compared with the ouabain-bound complex (2015), Proc. Natl. Acad. Sci. USA, 112, 1755-1760.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
7.2.2.13	E2P enzyme form in complex with inhibitors, hanging drop vapor diffusion method, using 17% (w/v) PEG 2000 monomethyl ether, 10% (v/v) glycerol, 220 mM MgCl2,100 mM KCl, 100 mM MES (titrated with KOH, pH 6.2), 5% (v/v) 2-methyl-2,4-pentanediol, and 6% (v/v) Jeffamine M-600 (titrated with KOH, pH 6.2)	Sus scrofa

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
7.2.2.13	bufalin	-	Sus scrofa
7.2.2.13	digitoxigenin	-	Sus scrofa
7.2.2.13	digoxin	-	Sus scrofa
7.2.2.13	ouabagenin	-	Sus scrofa
7.2.2.13	Ouabain	-	Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
7.2.2.13	ATP + H2O + Na+/in + K+/out	Sus scrofa	-	ADP + phosphate + Na+/out + K+/in	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
7.2.2.13	Sus scrofa	P05024	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
7.2.2.13	-	Sus scrofa

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
7.2.2.13	kidney	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.2.2.13	30	-	at pH 7.0 and 37°C	Sus scrofa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7.2.2.13	ATP + H2O + Na+/in + K+/out	-	Sus scrofa	ADP + phosphate + Na+/out + K+/in	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
7.2.2.13	Na+,K+-ATPase	-	Sus scrofa

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Release 2023.1 (January 2023)