BRENDA - Enzyme Database

Subcellular localization of monoglucosyldiacylglycerol synthase in Synechocystis sp. PCC6803 and its unique regulation by lipid environment

Selao, T.; Zhang, L.; Ariz, C.; Wieslander, A.; Norling, B.; PLoS ONE 9, e88153 (2014)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.46
phosphatidylethanolamine
mild activity-enhancing effect (60%, at 40 mol percent concentration)
Synechocystis sp.
2.4.1.46
sulfoquinovosyldiacylglycerol
most potent activator (100% increase of activity at 10 mol percent concentration)
Synechocystis sp.
2.4.1.336
sulfoquinovosyldiacylglycerol
7fold stimulation by 40 mol% in mixed micelles; potently stimulates the enzyme activity
Synechocystis sp.
Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.46
expressed in Escherichia coli BL21-DE3 Rosetta cells
Synechocystis sp.
2.4.1.336
expression in Escherichia coli
Synechocystis sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.46
digalactosyldiacylglycerol
-
Synechocystis sp.
2.4.1.46
monogalactosyldiacylglycerol
-
Synechocystis sp.
2.4.1.336
digalactosyldiacylglycerol
; inhibition by the final products of galactolipid synthesis
Synechocystis sp.
2.4.1.336
monogalactosyldiacylglycerol
; inhibition by the final products of galactolipid synthesis
Synechocystis sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.46
plasma membrane
-
Synechocystis sp.
5886
-
2.4.1.46
thylakoid membrane
-
Synechocystis sp.
42651
-
2.4.1.336
membrane
integral membrane protein; plasma and thylakoid membranes
Synechocystis sp.
16020
-
2.4.1.336
plasma membrane
-
Synechocystis sp.
5886
-
2.4.1.336
thylakoid
membrane
Synechocystis sp.
9579
-
2.4.1.336
thylakoid membrane
-
Synechocystis sp.
42651
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
Synechocystis sp.
-
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
?
2.4.1.336
additional information
Synechocystis sp.
production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase
?
-
-
-
2.4.1.336
UDP-glucose + 1,2-diacyl-sn-glycerol
Synechocystis sp.
-
UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.46
Synechocystis sp.
-
-
-
2.4.1.336
Synechocystis sp.
P74165
; gene sll1377
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.336
native enzyme from membranes fraction by ultracentrifugation
Synechocystis sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
735136
Synechocystis sp.
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
-
?
2.4.1.336
additional information
production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase
735136
Synechocystis sp.
?
-
-
-
-
2.4.1.336
UDP-glucose + 1,2-diacyl-sn-glycerol
-
735136
Synechocystis sp.
UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.336
30
-
assay at
Synechocystis sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.336
8
-
assay at
Synechocystis sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.46
phosphatidylethanolamine
mild activity-enhancing effect (60%, at 40 mol percent concentration)
Synechocystis sp.
2.4.1.46
sulfoquinovosyldiacylglycerol
most potent activator (100% increase of activity at 10 mol percent concentration)
Synechocystis sp.
2.4.1.336
sulfoquinovosyldiacylglycerol
7fold stimulation by 40 mol% in mixed micelles; potently stimulates the enzyme activity
Synechocystis sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.46
expressed in Escherichia coli BL21-DE3 Rosetta cells
Synechocystis sp.
2.4.1.336
expression in Escherichia coli
Synechocystis sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.46
digalactosyldiacylglycerol
-
Synechocystis sp.
2.4.1.46
monogalactosyldiacylglycerol
-
Synechocystis sp.
2.4.1.336
digalactosyldiacylglycerol
; inhibition by the final products of galactolipid synthesis
Synechocystis sp.
2.4.1.336
monogalactosyldiacylglycerol
; inhibition by the final products of galactolipid synthesis
Synechocystis sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.46
plasma membrane
-
Synechocystis sp.
5886
-
2.4.1.46
thylakoid membrane
-
Synechocystis sp.
42651
-
2.4.1.336
membrane
integral membrane protein; plasma and thylakoid membranes
Synechocystis sp.
16020
-
2.4.1.336
plasma membrane
-
Synechocystis sp.
5886
-
2.4.1.336
thylakoid
membrane
Synechocystis sp.
9579
-
2.4.1.336
thylakoid membrane
-
Synechocystis sp.
42651
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
Synechocystis sp.
-
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
?
2.4.1.336
additional information
Synechocystis sp.
production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase
?
-
-
-
2.4.1.336
UDP-glucose + 1,2-diacyl-sn-glycerol
Synechocystis sp.
-
UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.336
native enzyme from membranes fraction by ultracentrifugation
Synechocystis sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
735136
Synechocystis sp.
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
-
?
2.4.1.336
additional information
production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase
735136
Synechocystis sp.
?
-
-
-
-
2.4.1.336
UDP-glucose + 1,2-diacyl-sn-glycerol
-
735136
Synechocystis sp.
UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.336
30
-
assay at
Synechocystis sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.336
8
-
assay at
Synechocystis sp.
General Information
EC Number
General Information
Commentary
Organism
2.4.1.336
metabolism
the enzyme is involved in the lipid synthesis in Synechocystis, pathway overview
Synechocystis sp.
2.4.1.336
physiological function
synthesis of monogalactosyldiacylglycerol and digalactosyldiacylglycerol, the major membrane lipids in cyanobacteria, begins with production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase
Synechocystis sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.4.1.336
metabolism
the enzyme is involved in the lipid synthesis in Synechocystis, pathway overview
Synechocystis sp.
2.4.1.336
physiological function
synthesis of monogalactosyldiacylglycerol and digalactosyldiacylglycerol, the major membrane lipids in cyanobacteria, begins with production of the intermediate precursor monoglucosyldiacylglycerol, by monoglucosyldiacylglycerol synthase
Synechocystis sp.