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Literature summary extracted from

  • Gong, J.S.; Li, H.; Zhu, X.Y.; Lu, Z.M.; Wu, Y.; Shi, J.S.; Xu, Z.H.
    Fungal His-tagged nitrilase from Gibberella intermedia: gene cloning, heterologous expression and biochemical properties (2012), PLoS ONE, 7, e50622.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.1 EDTA 114% activity at 1 mM Fusarium proliferatum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.1 expressed in Escherichia coli Rosetta-gami (DE3) cells Fusarium proliferatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 Ag+ complete inhibition at 1 mM Fusarium proliferatum
3.5.5.1 Ba2+ about 76% residual activity at 1 mM Fusarium proliferatum
3.5.5.1 Ca2+ about 77% residual activity at 1 mM Fusarium proliferatum
3.5.5.1 Co2+ 1 mM decreases the enzyme activity to about 90% Fusarium proliferatum
3.5.5.1 Cu2+ about 75% residual activity at 1 mM Fusarium proliferatum
3.5.5.1 ethanol a relative activity of 49% is detected in 5% (v/v) of ethanol Fusarium proliferatum
3.5.5.1 Fe2+ about 79% residual activity at 1 mM Fusarium proliferatum
3.5.5.1 Li+ about 70% residual activity at 1 mM Fusarium proliferatum
3.5.5.1 methanol the enzyme is relatively active in 5% (v/v) methanol with 60% of the enzyme activity remaining Fusarium proliferatum
3.5.5.1 Ni2+ 1 mM decreases the enzyme activity to about 90% Fusarium proliferatum
3.5.5.1 Pb2+ about 78% residual activity at 1 mM Fusarium proliferatum
3.5.5.1 Propanol a relative activity of 6% is detected in 5% (v/v) of propanol Fusarium proliferatum
3.5.5.1 Zn2+ 1 mM decreases the enzyme activity to about 90% Fusarium proliferatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.1 12.11
-
 3-Cyanopyridine at pH 7.2 and 30°C Fusarium proliferatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.1 Mg2+ 110% activity at 1 mM Fusarium proliferatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 35940
-
 x * 35940, calculated from amino acid sequence Fusarium proliferatum
3.5.5.1 37000
-
 x * 37000, SDS-PAGE Fusarium proliferatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.1 3-cyanopyridine + 2 H2O Fusarium proliferatum 100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine nicotinic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O Fusarium proliferatum 69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine pyridine 4-carboxylic acid + NH3
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.5.5.1 additional information when 20% (v/v) of organic solvent is used, the enzyme activity sharply decreases and only can be detected with very low activity except that in methanol and 2-chloromethane (40% and 10% of activity retaining, respectively) Fusarium proliferatum

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Fusarium proliferatum
-
-
-
3.5.5.1 Fusarium proliferatum CA3-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1 Ni-NTA column chromatography Fusarium proliferatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2-chloro-3-cyanopyridine + H2O 0.21% activity compared to 3-cyanopyridine Fusarium proliferatum ?
-
 ?
3.5.5.1 2-chloro-4-cyanopyridine + H2O 7.48% activity compared to 3-cyanopyridine Fusarium proliferatum ?
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O 100% activity, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine Fusarium proliferatum nicotinic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O 69.25% activity compared to 3-cyanopyridine, the enzyme is highly specific towards heterocyclic nitriles such as 3- and 4-cyanopyridine Fusarium proliferatum pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.1 4-cyanopyridine + 2 H2O 0.07% activity compared to 3-cyanopyridine Fusarium proliferatum pyridine 4-carboxylic acid + NH3
-
 ?
3.5.5.1 acetonitrile + H2O 2.31% activity compared to 3-cyanopyridine Fusarium proliferatum acetate + NH3
-
 ?
3.5.5.1 acetonitrile + H2O 2.31% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 acetate + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O 43.96% activity compared to 3-cyanopyridine Fusarium proliferatum acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O 43.96% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 acrylic acid + NH3
-
 ?
3.5.5.1 adiponitrile + H2O 106.29% activity compared to 3-cyanopyridine Fusarium proliferatum adipic acid + NH3
-
 ?
3.5.5.1 adiponitrile + H2O 106.29% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 adipic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O 42.14% activity compared to 3-cyanopyridine Fusarium proliferatum benzoic acid + NH3
-
 ?
3.5.5.1 glycinonitrile + H2O 23.27% activity compared to 3-cyanopyridine Fusarium proliferatum ?
-
 ?
3.5.5.1 iminodiacetonitrile + 2 H2O 58.07% activity compared to 3-cyanopyridine Fusarium proliferatum iminodiacetic acid + 2 NH3
-
 ?
3.5.5.1 iminodiacetonitrile + 2 H2O 58.07% activity compared to 3-cyanopyridine Fusarium proliferatum CA3-1 iminodiacetic acid + 2 NH3
-
 ?
3.5.5.1 isovaleronitrile + H2O 47.17% activity compared to 3-cyanopyridine Fusarium proliferatum isovaleric acid + NH3
-
 ?
3.5.5.1 additional information no activity with mandelonitrile and geranyl nitrile Fusarium proliferatum ?
-
 ?
3.5.5.1 additional information no activity with mandelonitrile and geranyl nitrile Fusarium proliferatum CA3-1 ?
-
 ?
3.5.5.1 phenylacetonitrile + 2 H2O 21.59% activity compared to 3-cyanopyridine Fusarium proliferatum phenylacetate + NH3
-
 ?
3.5.5.1 succinonitrile + H2O 64.22% activity compared to 3-cyanopyridine Fusarium proliferatum succinic acid + NH3
-
 ?
3.5.5.1 valeronitrile + H2O 45.07% activity compared to 3-cyanopyridine Fusarium proliferatum valeric acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 ? x * 37000, SDS-PAGE Fusarium proliferatum
3.5.5.1 ? x * 35940, calculated from amino acid sequence Fusarium proliferatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 45
-
-
 Fusarium proliferatum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 20 50 the nitrilase activity gradually increases from 20°C to 45°C and is lost rapidly above 50°C Fusarium proliferatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7.8
-
-
 Fusarium proliferatum