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Literature summary extracted from

  • Bian, F.; Yue, S.; Peng, Z.; Zhang, X.; Chen, G.; Yu, J.; Xuan, N.; Bi, Y.
    A comprehensive alanine-scanning mutagenesis study reveals roles for salt bridges in the structure and activity of Pseudomonas aeruginosa elastase (2015), PLoS ONE, 10, e121108.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.26 recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.26 D168A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
3.4.24.26 D189A site-directed mutagenesis, the mutant shows decreased thermal stabilities and increased activities compared to the wild-type enzyme Pseudomonas aeruginosa
3.4.24.26 D201A site-directed mutagenesis, the mutant shows slightly increased thermal stability and slightly decreased activity compared to the wild-type enzyme Pseudomonas aeruginosa
3.4.24.26 E249A site-directed mutagenesis, the mutant shows both decreased thermal stability and decreased activity compared to the wild-type enzyme Pseudomonas aeruginosa
3.4.24.26 R179A site-directed mutagenesis, the mutant shows decreased thermal stabilities and increased activities compared to the wild-type enzyme Pseudomonas aeruginosa
3.4.24.26 R198A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
3.4.24.26 R205A site-directed mutagenesis, the mutant shows slightly increased thermal stability and slightly decreased activity compared to the wild-type enzyme Pseudomonas aeruginosa
3.4.24.26 R245A site-directed mutagenesis Pseudomonas aeruginosa
3.4.24.26 R253A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa
3.4.24.26 R279A site-directed mutagenesis, inactive mutant Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.26 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.26 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by ammonium sulfate fractionation, anion exchange chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.26 3-(2-furylacryloyl)glycyl-L-leucine amide + H2O
-
 Pseudomonas aeruginosa ?
-
 ?
3.4.24.26 casein + H2O
-
 Pseudomonas aeruginosa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.26 elastase
-
 Pseudomonas aeruginosa
3.4.24.26 PAE
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.26 30
-
-
 Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.26 8
-
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
3.4.24.26 evolution the enzyme is an M4 family metalloprotease Pseudomonas aeruginosa
3.4.24.26 additional information salt bidges in the enzyme, structure overview. Relationship between salt bridges and stability/enzymatic activity, structure analysis and molecular dynamics using crystal structure with PDB ID 1EZM, overview Pseudomonas aeruginosa