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Literature summary extracted from

  • Luo, J.; Fuell, C.; Parr, A.; Hill, L.; Bailey, P.; Elliott, K.; Fairhurst, S.A.; Martin, C.; Michael, A.J.
    A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed (2009), Plant Cell, 21, 318-333.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.248 gene At2g23510, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS Arabidopsis thaliana
2.3.1.249 gene At2g25150, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.248 additional information construction of a T-DNA insertion mutant SM_3_38374, complementation of At2g23510 knockout mutant phenotype by overexpression of wild-type gene At2g23510 under control of CMV 35S promoter using Agrobacterium tumefaciens strain GV3101 transfrmation method. Transcript of At2g23510 can be detected in siliques from wild-type plants but not in those from the insertion line, confirming that the insertion eliminates production of the At2g23510 transcript Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.248 0.0083
-
sinapoyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 0.0346
-
N1,N8-bis(sinapoyl)-spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 0.0374
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 0.0836
-
CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 0.2369
-
putrescine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.249 0.0106
-
4-coumaroyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.249 0.0527
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.248 2 sinapoyl-CoA + spermidine Arabidopsis thaliana
-
2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
2.3.1.248 2 sinapoyl-CoA + spermidine Arabidopsis thaliana Col-0
-
2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
2.3.1.249 2 4-coumaroyl-CoA + spermidine Arabidopsis thaliana the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2.3.1.249 2 4-coumaroyl-CoA + spermidine Arabidopsis thaliana Col-0 the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.248 Arabidopsis thaliana O80467 gene At2g23510
-
2.3.1.248 Arabidopsis thaliana Col-0 O80467 gene At2g23510
-
2.3.1.249 Arabidopsis thaliana Q8GYW8 gene At2g25150
-
2.3.1.249 Arabidopsis thaliana Col-0 Q8GYW8 gene At2g25150
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.248 recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration Arabidopsis thaliana
2.3.1.249 recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.248 seed the enzyme is expressed mainly in seeds Arabidopsis thaliana
-
2.3.1.248 seedling
-
Arabidopsis thaliana
-
2.3.1.248 silique
-
Arabidopsis thaliana
-
2.3.1.249 root the enzyme is expressed specifically in roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.248 2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
Arabidopsis thaliana 2 sinapoyl-CoA + spermidine
-
r
2.3.1.248 2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
Arabidopsis thaliana Col-0 2 sinapoyl-CoA + spermidine
-
r
2.3.1.248 2 sinapoyl-CoA + putrescine
-
Arabidopsis thaliana ?
-
r
2.3.1.248 2 sinapoyl-CoA + putrescine
-
Arabidopsis thaliana Col-0 ?
-
r
2.3.1.248 2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana 2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
r
2.3.1.248 2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana 2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
2.3.1.248 2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(sinapoyl)-spermidine
-
r
2.3.1.248 2 sinapoyl-CoA + spermidine
-
Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(sinapoyl)-spermidine identification of two derivatives, i.e. N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N1,N8-di(sinapoyl)-spermidine, in seeds by NMR analysis r
2.3.1.248 additional information no activity with acyl donors coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors cadaverine, norspermidine, spermine, homospermidine, agmatine, and tyramine, low activity with putrescine Arabidopsis thaliana ?
-
?
2.3.1.248 additional information no activity with acyl donors coumaroyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors cadaverine, norspermidine, spermine, homospermidine, agmatine, and tyramine, low activity with putrescine Arabidopsis thaliana Col-0 ?
-
?
2.3.1.249 2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo Arabidopsis thaliana 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2.3.1.249 2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo and is absolutely specific for 4-coumaroyl-CoA and spermidine Arabidopsis thaliana 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2.3.1.249 2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2.3.1.249 2 4-coumaroyl-CoA + spermidine the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo and is absolutely specific for 4-coumaroyl-CoA and spermidine Arabidopsis thaliana Col-0 2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
-
?
2.3.1.249 additional information no activity with acyl donors sinapoyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors putrescine, cadaverine, spermine, agmatine, and tyramine Arabidopsis thaliana ?
-
?
2.3.1.249 additional information no activity with acyl donors sinapoyl-CoA, caffeoyl-CoA, and feruloyl-CoA, and also no activity with acyl acceptors putrescine, cadaverine, spermine, agmatine, and tyramine Arabidopsis thaliana Col-0 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.248 BAHD acyl transferase
-
Arabidopsis thaliana
2.3.1.248 SDT
-
Arabidopsis thaliana
2.3.1.248 spermidine sinapoyl CoA acyltransferase
-
Arabidopsis thaliana
2.3.1.249 BAHD acyl transferase
-
Arabidopsis thaliana
2.3.1.249 SCT
-
Arabidopsis thaliana
2.3.1.249 spermidine coumaroyl CoA acyltransferase
-
Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.248 30
-
assay at Arabidopsis thaliana
2.3.1.249 30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.248 0.3
-
putrescine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 5.1
-
sinapoyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 5.6
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 37.8
-
N1,N8-bis(sinapoyl)-spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.248 39.6
-
CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.249 3.1
-
spermidine pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana
2.3.1.249 3.4
-
4-coumaroyl-CoA pH 7.3, 30°C, recombinant His6-tagged enzyme Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.248 8
-
recombinant enzyme, reverse reaction Arabidopsis thaliana
2.3.1.248 9
-
recombinant enzyme, forward reaction Arabidopsis thaliana
2.3.1.249 7.3
-
assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.3.1.248 evolution the enzyme is a member of the BAHD acyltransferase family Arabidopsis thaliana
2.3.1.248 malfunction enzyme T-DNA Insertion knockout mutant SM_3_38374 phenotype, metabolic profiling, overview Arabidopsis thaliana
2.3.1.248 physiological function spermidine sinapoyl CoA acyltransferase is required for the production of disinapoyl spermidine and its glucoside in Arabidopsis seeds. The sinapoyl spermidine derivatives, N-((4'-O-glycosyl)-sinapoyl),N'-sinapoylspermidine and N,N'-disinapoylspermidine, are the two major polyamine conjugates that accumulate in Arabidopsis thaliana seeds, metabolic profiling Arabidopsis thaliana
2.3.1.249 evolution the enzyme is a member of the BAHD acyltransferase family Arabidopsis thaliana