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Literature summary extracted from

  • Zhang, Q.; Marsolais, F.
    Identification and characterization of omega-amidase as an enzyme metabolically linked to asparagine transamination in Arabidopsis (2014), Phytochemistry, 99, 36-43.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.3 gene At5g12040, DNA and amino acid sequence determination and analysis, expression of N-terminally His-tagged recombinant enzyme lacking the chloroplast transit peptide, commencing at Met63, in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.3 4.43
-
 2-Oxoglutaramate pH 8.5, 30°C, recombinant His-tagged enzyme Arabidopsis thaliana
3.5.1.3 5.58
-
 2-hydroxysuccinamate pH 8.5, 30°C, recombinant His-tagged enzyme Arabidopsis thaliana
3.5.1.3 6.13
-
 2-oxosuccinamate pH 8.5, 30°C, recombinant His-tagged enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.3 chloroplast the enzyme contains a chloroplast transit peptide of 60 residues at the N-terminus Arabidopsis thaliana 9507
-
3.5.1.3 additional information second methionine residue in the deduced amino acid sequence, Met63, is located two residues C-terminal to the predicted cleavage site of the chloroplast transit peptide, N-terminal to Ser61. Alternative translation initiation from the corresponding downstream start codon can produce a cytosolic isoform, lacking the chloroplast transit peptide Arabidopsis thaliana
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.3 34000
-
 x * 40300, about, sequence calculation, x * 34000, recombinant His-tagged enzyme without transit peptide, SDS-PAGe Arabidopsis thaliana
3.5.1.3 40300
-
 x * 40300, about, sequence calculation, x * 34000, recombinant His-tagged enzyme without transit peptide, SDS-PAGe Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.3 2-oxosuccinamate + H2O Arabidopsis thaliana the product of asparagine transamination, 2-oxosuccinamate, can be hydrolyzed by the enzyme omega-amidase to form oxaloacetate and ammonia oxaloacetate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O Arabidopsis thaliana Col the product of asparagine transamination, 2-oxosuccinamate, can be hydrolyzed by the enzyme omega-amidase to form oxaloacetate and ammonia oxaloacetate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.3 Arabidopsis thaliana Q8RUF8 gene At5g12040
-
3.5.1.3 Arabidopsis thaliana Col Q8RUF8 gene At5g12040
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.3 recombinant N-terminally His-tagged enzyme lacking the chloroplast transit peptide from Escherichia coli by nickel affinity chromatography and desalting gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.3 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.3 2-hydroxysuccinamate + H2O
-
 Arabidopsis thaliana malate + NH3
-
 ?
3.5.1.3 2-hydroxysuccinamate + H2O
-
 Arabidopsis thaliana Col malate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + H2O the product of glutamine transamination, 2-oxoglutaramate, can be hydrolyzed by the enzyme omega-amidase to form and ammonia Arabidopsis thaliana 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxoglutaramate + H2O the product of glutamine transamination, 2-oxoglutaramate, can be hydrolyzed by the enzyme omega-amidase to form and ammonia Arabidopsis thaliana Col 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O
-
 Arabidopsis thaliana oxaloacetate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O the product of asparagine transamination, 2-oxosuccinamate, can be hydrolyzed by the enzyme omega-amidase to form oxaloacetate and ammonia Arabidopsis thaliana oxaloacetate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O
-
 Arabidopsis thaliana Col oxaloacetate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O the product of asparagine transamination, 2-oxosuccinamate, can be hydrolyzed by the enzyme omega-amidase to form oxaloacetate and ammonia Arabidopsis thaliana Col oxaloacetate + NH3
-
 ?
3.5.1.3 additional information the recombinant omega-amidase is equally active with 2-oxosuccinamate, 2-oxoglutaramate and 2-hydroxysuccinamate Arabidopsis thaliana ?
-
 ?
3.5.1.3 additional information the recombinant omega-amidase is equally active with 2-oxosuccinamate, 2-oxoglutaramate and 2-hydroxysuccinamate Arabidopsis thaliana Col ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.3 ? x * 40300, about, sequence calculation, x * 34000, recombinant His-tagged enzyme without transit peptide, SDS-PAGe Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.3 8.5
-
 assay at Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
3.5.1.3 metabolism omega-amidase is involved in the metabolism of asparagine, and probably is also closely coupled with glutamine transamination in the methionine salvage cycle Arabidopsis thaliana