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Literature summary extracted from

  • Lee, J.; Kim, J.H.; Sohn, H.J.; Yang, H.J.; Na, B.K.; Chwae, Y.J.; Park, S.; Kim, K.; Shin, H.J.
    Novel cathepsin B and cathepsin B-like cysteine protease of Naegleria fowleri excretory-secretory proteins and their biochemical properties (2014), Parasitol. Res., 113, 2765-2776.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.1 expressed as a His-tagged fusion protein in Escherichia coli Naegleria fowleri

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 E-64
-
 Naegleria fowleri
3.4.22.1 E-64d
-
 Naegleria fowleri
3.4.22.1 IAA
-
 Naegleria fowleri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.0287
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 0.0358
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 0.0608
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 0.0656
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 34000
-
 calculated from cDNA Naegleria fowleri
3.4.22.1 38400
-
 calculated from cDNA Naegleria fowleri

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Naegleria fowleri X5D761
-
-
3.4.22.1 Naegleria fowleri X5D911
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1 using affinity chromatography Naegleria fowleri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 additional information expression is detected in the trophozoite stage but not in the cyst stage Naegleria fowleri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 Albumin + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 Collagen + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 Fibronectin + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 Hemoglobin + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 IgA + H2O
-
 Naegleria fowleri ?
-
 ?
3.4.22.1 IgG + H2O
-
 Naegleria fowleri ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.1 cathepsin B
-
 Naegleria fowleri
3.4.22.1 nfcpb
-
 Naegleria fowleri
3.4.22.1 nfcpb-L
-
 Naegleria fowleri

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.1 0.318
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 0.327
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 0.476
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 0.512
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 4.5
-
-
 Naegleria fowleri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.1 4.5 6.5
-
 Naegleria fowleri

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.1 4 8
-
 Naegleria fowleri
3.4.22.1 4 7
-
 Naegleria fowleri

Expression

EC Number Organism Comment Expression
3.4.22.1 Naegleria fowleri expression is detected in the trophozoite stage but not in the cyst stage up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.22.1 4.82
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 5.38
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 13.31
-
 benzyloxycarbonyl-L-phenylalaninyl-L-arginine 4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri
3.4.22.1 17.86
-
 benzyloxycarbonyl-L-leucyl-L-arginine-4-methylcoumaryl-7-amide pH 4.5,37°C Naegleria fowleri