Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Song, H.; Her, A.S.; Raso, F.; Zhen, Z.; Huo, Y.; Liu, P.
    Cysteine oxidation reactions catalyzed by a mononuclear non-heme iron enzyme (OvoA) in ovothiol biosynthesis (2014), Org. Lett., 16, 2122-2125.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.99.52 synthesis potential application of the oxidative coupling between hercynine and cysteine for industrial ergothioneine production Erwinia tasmaniensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.52 gene ovoA, the mutant enzyme loses the cysteine dioxygenase activity but also stops catalyzing the formation of oxidative coupling product Erwinia tasmaniensis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.99.52 E176H site-directed mutagenesis Erwinia tasmaniensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.52 Fe2+ a mononuclear non-heme iron enzyme, dependent on, ligand with 2-His-1-carboxylate facial triad Erwinia tasmaniensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.52 L-histidine + L-cysteine + O2 Erwinia tasmaniensis
-
 S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
 ?
1.14.99.52 L-histidine + L-cysteine + O2 Erwinia tasmaniensis DSM 17950
-
 S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.52 Erwinia tasmaniensis B2VFD8 gene ovoA
-
1.14.99.52 Erwinia tasmaniensis DSM 17950 B2VFD8 gene ovoA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.52 hercynine + L-cysteine + O2
-
 Erwinia tasmaniensis S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.14.99.52 hercynine + L-cysteine + O2
-
 Erwinia tasmaniensis DSM 17950 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
1.14.99.52 L-cysteine + O2 oxidation of cysteine to cysteine sulfinic acid in presence of hercynine or L-histidine Erwinia tasmaniensis cysteine sulfinic acid + H2O
-
 ?
1.14.99.52 L-cysteine + O2 oxidation of cysteine to cysteine sulfinic acid in presence of hercynine or L-histidine Erwinia tasmaniensis DSM 17950 cysteine sulfinic acid + H2O
-
 ?
1.14.99.52 L-cysteine + O2 oxidation of cysteine to cystine Erwinia tasmaniensis L-cystine + H2O
-
 ?
1.14.99.52 L-cysteine + O2 oxidation of cysteine to cystine Erwinia tasmaniensis DSM 17950 L-cystine + H2O
-
 ?
1.14.99.52 L-histidine + L-cysteine + O2
-
 Erwinia tasmaniensis S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
 ?
1.14.99.52 L-histidine + L-cysteine + O2
-
 Erwinia tasmaniensis DSM 17950 S-(L-histidin-5-yl)-L-cysteine S-oxide + H2O
-
 ?
1.14.99.52 additional information the enzyme catalyzes the oxidative coupling between histidine and cysteine. It can also catalyze the oxidative coupling between hercynine and cysteine, yet with a different regioselectivity, NMR spectroscopic product analysis, overview. Enzyme OvoA can also catalyze the oxidation of cysteine to either cysteine sulfinic acid or cystine. OvoA-catalyzed reactions can be systematically modulated by a slight modification of one of its substrates, histidine Erwinia tasmaniensis ?
-
 ?
1.14.99.52 additional information the enzyme catalyzes the oxidative coupling between histidine and cysteine. It can also catalyze the oxidative coupling between hercynine and cysteine, yet with a different regioselectivity, NMR spectroscopic product analysis, overview. Enzyme OvoA can also catalyze the oxidation of cysteine to either cysteine sulfinic acid or cystine. OvoA-catalyzed reactions can be systematically modulated by a slight modification of one of its substrates, histidine Erwinia tasmaniensis DSM 17950 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.52 5-histidylcysteine sulfoxide synthase
-
 Erwinia tasmaniensis
1.14.99.52 OvoA
-
 Erwinia tasmaniensis

General Information

EC Number General Information Comment Organism
1.14.99.52 evolution enzyme OvoA belongs to the 2-His-1-carboxylate catalytic triad type of mononuclear non-heme iron enzymes Erwinia tasmaniensis
1.14.99.52 malfunction mutation of the 2-His-1-carboxylate catalytic triad of the enzyme disrupts the cysteine dioxygenase activity Erwinia tasmaniensis
1.14.99.52 metabolism OvoAis required in ovothiol biosynthesis catalyzing the oxidative coupling between histidine and cysteine Erwinia tasmaniensis