Any feedback?
Literature summary extracted from
- Human dCTP pyrophosphatase 1 promotes breast cancer cell growth and stemness through the modulation on 5-methyl-dCTP metabolism and global hypomethylation (2015), Oncogenesis, 4, e159.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.6.1.12 | generation of 3D models of DCTPP1 and its 5-methyl-dCTP-bound tetramer based on X-ray crystal structure of the tetrameric mouse dCTPase 1, PDB entry 2OIG. The cytosines of substrates 5-methyl-dCTP and dCTP bind deeply into the substrate pocket, which is composed of the residues from the dimer interface, including His38, Trp47, Glu63, Glu66, Trp73, Glu95, Asp98 and Tyr102 | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.12 | 0.085 | - |
5-Methyl-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens |  |
| 3.6.1.12 | 0.0944 | - |
5-iodo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.109 | - |
5-bromo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.152 | - |
dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.196 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.24 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.293 | - |
dATP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.12 | Homo sapiens | Q9H773 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.6.1.12 | MCF-7 cell | - |
Homo sapiens | - |
| 3.6.1.12 | MDA-MB-231 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.12 | 5-bromo-dCTP + H2O | - |
Homo sapiens | 5-bromo-dCMP + diphosphate | - |
? | |
| 3.6.1.12 | 5-iodo-dCTP + H2O | - |
Homo sapiens | 5-iodo-dCMP + diphosphate | - |
? | |
| 3.6.1.12 | 5-methyl-dCTP + H2O | - |
Homo sapiens | 5-methyl-dCMP + diphosphate | - |
? | |
| 3.6.1.12 | dATP + H2O | - |
Homo sapiens | dAMP + diphosphate | - |
? | |
| 3.6.1.12 | dCTP + H2O | - |
Homo sapiens | dCMP + diphosphate | - |
? | |
| 3.6.1.12 | dTTP + H2O | - |
Homo sapiens | dTMP + diphosphate | - |
? | |
| 3.6.1.12 | dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
| 3.6.1.12 | additional information | no substrate: dGTP | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.12 | 0.081 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.108 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.14 | - |
dATP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.362 | - |
5-Methyl-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.367 | - |
dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.43 | - |
5-iodo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.505 | - |
5-bromo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.12 | 7.8 | 11 | more than 80% of maximum activity | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.6.1.12 | physiological function | knockdown of DCTPP1 in breast cancer cell line MCF-7 cells remarkably retards proliferation and colony formation in vitro. The capacity of mammosphere formation of MCF-7 is suppressed with the silence of DCTPP1, consistent with the enhanced mammosphere-forming ability in DCTPP1-overexpressing MDA-MB-231 cells. The global methylation level is elevated in DCTPP1-knockdown MCF-7 cells or mammosphere-forming MCF-7 cells but decreased significantly in DCTPP1-overexpressing MDA-MB-231 cells and its mammosphere | Homo sapiens |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.12 | 0.337 | - |
dUTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.483 | - |
dATP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 0.551 | - |
dTTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 2.4 | - |
dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 4.25 | - |
5-Methyl-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 4.56 | - |
5-iodo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
| 3.6.1.12 | 4.63 | - |
5-bromo-dCTP | pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
