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Literature summary extracted from

  • Shoji, T.; Takaya, A.; Sato, Y.; Kimura, S.; Suzuki, T.; Yamamoto, T.
    RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAII in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility (2015), Nucleic Acids Res., 43, 8964-8972.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.188 additional information methylation of the adjacent uridine at position 747 by RlmCD enhances guanine748 methylation by the enzyme Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.188 expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.188 32000
-
 x * 32000, SDS-PAGE Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus pneumoniae
-
 S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus pneumoniae S1
-
 S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.189 additional information Streptococcus pneumoniae the enzyme also methylates uracil1939 in 23S rRNA ?
-
 ?
2.1.1.189 additional information Streptococcus pneumoniae S1 the enzyme also methylates uracil1939 in 23S rRNA ?
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA Streptococcus pneumoniae
-
 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA Streptococcus pneumoniae S1
-
 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.190 additional information Streptococcus pneumoniae the enzyme also methylates uracil747 in 23S rRNA ?
-
 ?
2.1.1.190 additional information Streptococcus pneumoniae S1 the enzyme also methylates uracil747 in 23S rRNA ?
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA Streptococcus pneumoniae
-
 S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA Streptococcus pneumoniae S1
-
 S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.188 Streptococcus pneumoniae
-
-
-
2.1.1.188 Streptococcus pneumoniae S1
-
-
-
2.1.1.189 Streptococcus pneumoniae
-
-
-
2.1.1.189 Streptococcus pneumoniae S1
-
-
-
2.1.1.190 Streptococcus pneumoniae
-
-
-
2.1.1.190 Streptococcus pneumoniae S1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.188 glutathione Sepharose column chromatography and Superdex 75 gel filtration Streptococcus pneumoniae
2.1.1.188 glutathione Sepharose column chromatography and Superdex75 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
 Streptococcus pneumoniae S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.188 S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
 Streptococcus pneumoniae S1 S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
 ?
2.1.1.189 additional information the enzyme also methylates uracil1939 in 23S rRNA Streptococcus pneumoniae ?
-
 ?
2.1.1.189 additional information the enzyme also methylates uracil1939 in 23S rRNA Streptococcus pneumoniae S1 ?
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
 Streptococcus pneumoniae S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.189 S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
 Streptococcus pneumoniae S1 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
2.1.1.190 additional information the enzyme also methylates uracil747 in 23S rRNA Streptococcus pneumoniae ?
-
 ?
2.1.1.190 additional information the enzyme also methylates uracil747 in 23S rRNA Streptococcus pneumoniae S1 ?
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA
-
 Streptococcus pneumoniae S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?
2.1.1.190 S-adenosyl-L-methionine + uracil1939 in 23S rRNA
-
 Streptococcus pneumoniae S1 S-adenosyl-L-homocysteine + 5-methyluracil1939 in 23S rRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.188 ? x * 32000, SDS-PAGE Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
2.1.1.188 RlmAII
-
 Streptococcus pneumoniae
2.1.1.189 RlmCD
-
 Streptococcus pneumoniae
2.1.1.189 SP 1029
-
 Streptococcus pneumoniae
2.1.1.190 RlmCD
-
 Streptococcus pneumoniae
2.1.1.190 SP 1029
-
 Streptococcus pneumoniae

Expression

EC Number Organism Comment Expression
2.1.1.188 Streptococcus pneumoniae RlmCD disruption does not influence the level of the enzyme additional information

General Information

EC Number General Information Comment Organism
2.1.1.188 metabolism the enzyme enhances binding of telithromycin to A752 in ribosomes Streptococcus pneumoniae
2.1.1.189 malfunction enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B) Streptococcus pneumoniae
2.1.1.189 metabolism methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility Streptococcus pneumoniae