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Literature summary extracted from
- Non-redundant contribution of the plastidial FAD8 omega-3 desaturase to glycerolipid unsaturation at different temperatures in Arabidopsis (2015), Mol. Plant, 8, 1599-1611.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.35 | gene FAD7, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants | Arabidopsis thaliana |
| 1.14.19.35 | gene FAD8, DNA and amino acid sequence determination and analysis, genotyping of wild-type and mutants. Recombinant expression of AtFAD8-YFP fusion protein in stable transgenic Arabidopsis thaliana lines and transiently in Nicotinana benthamiana leaves, overexpression of YFP-tagged fad8 in Arabidosis thaliana results in increased 18:3 trienoic fatty acid levels in phospholipids but not in galactolipids | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.19.35 | C103Y | mutation occuring in the fad3 fad7 fad8 triple mutant | Arabidopsis thaliana |
| 1.14.19.35 | additional information | construction of a tandem T-DNA FAD7 insertion omega-3 desaturase mutant line SALK_147096C/fad7i. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable | Arabidopsis thaliana |
| 1.14.19.35 | additional information | construction of a tandem T-DNA FAD8 insertion omega-3 desaturase mutant line SALK_093590 with no phenotype at 22°C. Construction of double fad7/fad8 and triple fad3/fad7/fad8 mutants. FAD8-YFP overexpressing lines show a specific increase in 18:3 fatty acids at 22°C. Fatty acid composition of total leaf lipids from wild-type and the different mutant lines grown at 22°C, overview. Disruption of the AtFAD8 gene in mutant fad8i results in trienoic fatty acid levels almost similar to those from wild-type Col-0. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable | Arabidopsis thaliana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.35 | chloroplast envelope | enzymes FAD8 and FAD7 might be located in close vicinity in the envelope membrane | Arabidopsis thaliana | 9941 | - |
| 1.14.19.35 | membrane | - |
Arabidopsis thaliana | 16020 | - |
| 1.14.19.35 | additional information | localization study of recombinantly expressed fluorescence-tagged enzymes, overview | Arabidopsis thaliana | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.35 | a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Arabidopsis thaliana | - |
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |  |
| 1.14.19.35 | a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Arabidopsis thaliana Col-0 | - |
a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Arabidopsis thaliana | - |
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Arabidopsis thaliana Col-0 | - |
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | additional information | Arabidopsis thaliana | enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied | ? | - |
? | |
| 1.14.19.35 | additional information | Arabidopsis thaliana Col-0 | enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.35 | Arabidopsis thaliana | P46310 | gene fad7 | - |
| 1.14.19.35 | Arabidopsis thaliana | P48622 | gene FAD8 | - |
| 1.14.19.35 | Arabidopsis thaliana Col-0 | P46310 | gene fad7 | - |
| 1.14.19.35 | Arabidopsis thaliana Col-0 | P48622 | gene FAD8 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.35 | leaf | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.35 | a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Arabidopsis thaliana | a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | a (7Z,10Z)-hexadeca-7,10-dienoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Arabidopsis thaliana Col-0 | a (7Z,10Z,13Z)-hexadeca-7,10,13-trienoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Arabidopsis thaliana | an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Arabidopsis thaliana Col-0 | an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| 1.14.19.35 | additional information | enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied | Arabidopsis thaliana | ? | - |
? | |
| 1.14.19.35 | additional information | enzyme FAD8 has a higher selectivity for 18:2 acyl-lipid substrates and a higher preference for lipids other than galactolipids, particularly phosphatidylglycerol, at any of the temperatures studied | Arabidopsis thaliana Col-0 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.35 | FAD7 | - |
Arabidopsis thaliana |
| 1.14.19.35 | FAD8 | - |
Arabidopsis thaliana |
| 1.14.19.35 | omega-3 desaturase | - |
Arabidopsis thaliana |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.19.35 | 8 | 30 | active at | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.35 | Ferredoxin | required | Arabidopsis thaliana |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.19.35 | malfunction | a fad8 mutant shows no significant changes in its fatty acid profile at a control (22°C) temperature, while at lower temperature (15°C) the mutant shows a phenotype, leaf lipid analysis, overview | Arabidopsis thaliana |
| 1.14.19.35 | malfunction | mutant fad7i maintains 74% of 18:3 production with respect to Col-0 while only 23% of 16:3 synthesis is maintained in this mutant. The fad7/fad8 double mutant also shows a considerable reduction in trienoic fatty acids, particularly in 16:3, which is undetectable. After disruption of the AtFAD7 gene, enzyme FAD8 enzymatic activity is able to maintain, at least partially (43.7%), the amount of 18:3 and to a much lesser extent that of 16:3 (23.2%) at 22°C | Arabidopsis thaliana |
| 1.14.19.35 | additional information | FAD8-YFP over-expressing lines show a specific increase in 18:3 fatty acids at 22°C. Residue 103 is part of the first His box essential for desaturase function | Arabidopsis thaliana |
| 1.14.19.35 | physiological function | plastidial omega-3 desaturases FAD7 and FAD8 are major contributors to trienoic fatty acid biosynthesis in the leaves of Arabidopsis thaliana plants. Enzyme FAD8 partially compensates the disruption of the AtFAD7 gene at 22°C | Arabidopsis thaliana |
| 1.14.19.35 | physiological function | plastidial omega-3 desaturases FAD7 and FAD8 are major contributors to trienoic fatty acid biosynthesis in the leaves of Arabidopsis thaliana plants. Enzyme FAD8 partially compensates the disruption of the AtFAD7 gene at 22°C, indicating that enzyme FAD8 is active at this growth temperature, contrasting to previous observations that circumscribe the FAD8 activity at low temperatures | Arabidopsis thaliana |
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