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Literature summary extracted from

  • Hussain, A.; Ogawa, T.; Saito, M.; Sekine, T.; Nameki, M.; Matsushita, Y.; Hayashi, T.; Katayama, Y.
    Cloning and expression of a gene encoding a novel thermostable thiocyanate-degrading enzyme from a mesophilic Alphaproteobacteria strain THI201 (2013), Microbiology, 159, 2294-2302.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.5.8 thiocyanate maximum activity at 20-40 mM thiocyanate Alphaproteobacteria

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.8 expressed in Escherichia coli Rosetta-gami B and BL21(DE3) cells Alphaproteobacteria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.8 50000
-
 x * 50000, SDS-PAGE Alphaproteobacteria
3.5.5.8 50267
-
 x * 50267, calculated from amino acid sequence Alphaproteobacteria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.8 thiocyanate + H2O Alphaproteobacteria
-
 carbonyl sulfide + NH3 + HO-
-
 ?
3.5.5.8 thiocyanate + H2O Alphaproteobacteria THI201
-
 carbonyl sulfide + NH3 + HO-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.8 Alphaproteobacteria
-
-
-
3.5.5.8 Alphaproteobacteria THI201
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.8 hydroxyapatite column chromatography (native enzyme) and glutathione Sepharose column chromatography (recombinant enzyme). The activity of the enzyme purified at room temperature is 1.4times that of the enzyme purified at 4°C Alphaproteobacteria

Storage Stability

EC Number Storage Stability Organism
3.5.5.8 -20°C, recombinant enzyme, 1 week, complete loss of activity (72% of the original activity is recovered by the heat treatment of 60°C for 15 min) Alphaproteobacteria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.8 thiocyanate + H2O
-
 Alphaproteobacteria carbonyl sulfide + NH3 + HO-
-
 ?
3.5.5.8 thiocyanate + H2O
-
 Alphaproteobacteria THI201 carbonyl sulfide + NH3 + HO-
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.8 ? x * 50000, SDS-PAGE Alphaproteobacteria
3.5.5.8 ? x * 50267, calculated from amino acid sequence Alphaproteobacteria

Synonyms

EC Number Synonyms Comment Organism
3.5.5.8 SCNase
-
 Alphaproteobacteria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.8 30 70
-
 Alphaproteobacteria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.8 30 70
-
 Alphaproteobacteria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.8 4
-
 incubating recombinant enzyme on ice for 3 h reduces the enzyme activity to 2% of the original activity and subsequent heating at 60°C for 15 min returns the activity to the original level Alphaproteobacteria
3.5.5.8 30 80 the activity of the recombinant enzyme is completely retained after heat treatment at temperatures from 30 to 70°C, but is decreased by heating at 80°C for 5 min to 80%, to 60% at 90°C, and to 50% at 100°C Alphaproteobacteria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.8 6 6.5
-
 Alphaproteobacteria