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Literature summary extracted from
- Characterization of a family I-liked alkaline-resistant inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus furiosus for rapid determination of sugar nucleotidyltransferase at high temperature (2013), J. Mol. Catal. B, 98, 15-20.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.1 | expressed in Escherichia coli BL21(DE3) cells | Pyrococcus furiosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Ca2+ | - |
Pyrococcus furiosus |  |
| 3.6.1.1 | Cu2+ | - |
Pyrococcus furiosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.1 | 0.173 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 1.37 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 3.12 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 3.32 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 3.47 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 3.56 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 3.61 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 3.63 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 4.13 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 4.43 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 5.01 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 6.09 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 11.8 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Fe3+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
| 3.6.1.1 | Mg2+ | best activating ion | Pyrococcus furiosus | |
| 3.6.1.1 | Mn2+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
| 3.6.1.1 | additional information | not activated by Zn2+ | Pyrococcus furiosus | |
| 3.6.1.1 | Ni2+ | activates to a lesser extent than Mg2+ | Pyrococcus furiosus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 21740 | - |
2 * 21740, calculated from amino acid sequence | Pyrococcus furiosus |
| 3.6.1.1 | 22000 | - |
2 * 22000, SDS-PAGE | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | diphosphate + H2O | Pyrococcus furiosus | most specific substrate | 2 phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.1 | Pyrococcus furiosus | Q8U438 | - |
- |
| 3.6.1.1 | Pyrococcus furiosus ATCC 43587 | Q8U438 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.1 | Ni-NTA column chromatography | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 0.0559 | - |
with diphosphate as substrate, at pH 9.5 and 95°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | ADP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | ADP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| 3.6.1.1 | ATP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | ATP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| 3.6.1.1 | CDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | CDP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| 3.6.1.1 | CTP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | CTP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| 3.6.1.1 | diphosphate + H2O | most specific substrate | Pyrococcus furiosus | 2 phosphate | - |
? | |
| 3.6.1.1 | GDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | GTP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | GTP + H2O | - |
Pyrococcus furiosus ATCC 43587 | ? | - |
? | |
| 3.6.1.1 | IDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | ITP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | triphosphate + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | UDP + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | UDP-glucose + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
| 3.6.1.1 | UTP + H2O | weakest substrate | Pyrococcus furiosus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.6.1.1 | homodimer | 2 * 22000, SDS-PAGE | Pyrococcus furiosus |
| 3.6.1.1 | homodimer | 2 * 21740, calculated from amino acid sequence | Pyrococcus furiosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.1 | inorganic pyrophosphatase | - |
Pyrococcus furiosus |
| 3.6.1.1 | PPase | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 95 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 80 | 100 | the enzyme activity increases sharply from 80°C to 95°C. A moderate decrease in activity is observed between 95 and 100°C | Pyrococcus furiosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 85 | 100 | the enzyme shows half-lives of about 6850, 2760 and 850 min at 85, 95 and 100°C in the presence of Mg2+, respectively, as well as 3940, 1900, and 490 min at 85, 95 and 100°C in the absence of Mg2+, respectively | Pyrococcus furiosus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.1 | 19.7 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 27.5 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 29.7 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 39.9 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 51.3 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 57.3 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 91.7 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 91.7 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 94.2 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 99.1 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 116 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 226 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 1230 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 9.5 | - |
- |
Pyrococcus furiosus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 7.5 | 11 | over 50% of the maximal activity is retained in moderately alkaline conditions (pH ranging from 7.5 to 11.0 at 95°C), while less than 15% of the maximal activity of the enzyme is retained at pH values over 12.0 | Pyrococcus furiosus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.1 | 4.44 | - |
ADP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 4.87 | - |
UDP-glucose | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 8.28 | - |
UDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 8.4 | - |
UTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 11.2 | - |
GDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 16.4 | - |
CDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 16.5 | - |
IDP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 22.2 | - |
ATP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 23.2 | - |
ITP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 25.2 | - |
GTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 26.1 | - |
CTP | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 164 | - |
Triphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
| 3.6.1.1 | 7100 | - |
diphosphate | at pH 9.5 and 95°C | Pyrococcus furiosus | |
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