EC Number | Organism | UniProt | Comment | Textmining |
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3.4.22.3 | Ficus carica | - |
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EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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3.4.22.3 | 1-methylpiperidin-4-one + 4-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 44%, 19:81 anti/syn ratio, enantioselectivity: 5%/1% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | cyclohexanone + 3-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 39%, 86:14 anti/syn ratio, enantioselectivity: 81%/14% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | cyclohexanone + 4-cyanobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 79:21 anti/syn ratio, enantioselectivity: 77%/5% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | oxan-2-one + 4-bromobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 77:23 anti/syn ratio, enantioselectivity: 71%/27% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | oxan-2-one + 4-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 21%, 42:58 anti/syn ratio, enantioselectivity: 60%/24% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | oxan-3-one + 4-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 28%, 54:46 anti/syn ratio, enantioselectivity: 57%/28% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | oxan-4-one + 4-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 53:47 anti/syn ratio, enantioselectivity: 25%/17% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | tert-butyl 4-oxopiperidine-1-carboxylate + 3-bromobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 25%, 47:53 anti/syn ratio, enantioselectivity: 51%/45% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | tert-butyl 4-oxopiperidine-1-carboxylate + 3-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 29%, 51:49 anti/syn ratio, enantioselectivity: 59%/45% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | tert-butyl 4-oxopiperidine-1-carboxylate + 4-bromobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 35%, 75:25 anti/syn ratio, enantioselectivity: 67%/36% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 36%, 41:59 anti/syn ratio, enantioselectivity: 60%/14% (anti/syn) | Ficus carica | tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate | - |
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3.4.22.3 | tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde | the catalytic activity and stereoselectivity of ficin are significantly influenced by reaction media. Best yield of 34% with a poor enantioselectivity of 29% in THF. Best enantioselectivity of 57% with a yield of 28% in MeCN. In tested polar solvents including i-PrOH, DMSO, and DMF, ficin shows lower activityand enantioselectivity | Ficus carica | tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate | - |
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3.4.22.3 | tert-butyl 4-oxopiperidine-1-carboxylate + 4-nitrobenzaldehyde | water content is important for enantioselectivity and enzymatic activity. When water content is at 0.15 (H2O/(H2O+MeCN): 0.15,v/v) the reaction give the best enantioselectivity of 59% with 32% yield | Ficus carica | tert-butyl 3-[hydroxy(4-nitrophenyl)methyl]-4-oxopiperidine-1-carboxylate | - |
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3.4.22.3 | thian-4-one + 3-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 26%, 86:14 anti/syn ratio, enantioselectivity: 77%/1% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | thian-4-one + 4-cyanobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 23%, 84:16 anti/syn ratio, enantioselectivity: 76%/53% (anti/syn) | Ficus carica | ? | - |
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3.4.22.3 | thiolan-3-one + 4-nitrobenzaldehyde | reaction condition: 30°C, MeCN (0.85 mL) and deionized water (0.15 mL). Yield: 31%, 40:60 anti/syn ratio, enantioselectivity: 45%/23% (anti/syn) | Ficus carica | ? | - |
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EC Number | Synonyms | Comment | Organism |
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3.4.22.3 | ficin | - |
Ficus carica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
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3.4.22.3 | 25 | - |
assay at | Ficus carica |
3.4.22.3 | 30 | - |
best enantioselectivity of 60% at 30°C | Ficus carica |