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Literature summary extracted from

  • Nomme, J.; Su, Y.; Lavie, A.
    Elucidation of the specific function of the conserved threonine triad responsible for human L-asparaginase autocleavage and substrate hydrolysis (2014), J. Mol. Biol., 426, 2471-2485.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 glycine the enzyme is activated by incubating for 4 days in a 2.0 M glycine (pH 9.0) solution at room temperature Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expressed in Escherichia coli BL21(DE3) C41 cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 hanging drop vapor diffusion method, using 2.2-2.5 M sodium malonate (pH 7.0) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.1 T168S the mutant shows no self-cleavage Homo sapiens
3.5.1.1 T186V the inactive mutant shows partial (45%) self-cleavage Homo sapiens
3.5.1.1 T219A the inactive mutant shows a complete, albeit slow self-cleavage Homo sapiens
3.5.1.1 T219V the inactive mutant displays partial (50%) self-cleavage Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.12
-
 beta-L-Asp-L-Phe wild type enzyme, at pH 7.5 and 37°C Homo sapiens
3.5.1.1 2.09
-
 L-asparagine wild type enzyme, at pH 7.5 and 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.1 12000
-
 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE Homo sapiens
3.5.1.1 20000
-
 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE Homo sapiens
3.5.1.1 37000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Homo sapiens
-
 L-aspartate + NH3
-
 ?
3.5.1.1 additional information Homo sapiens to become enzymatically active, ASNase3 must undergo autocleavage between residues Gly167 and Thr168 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Homo sapiens Q7L266
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 nickel affinity column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 beta-L-Asp-L-Phe + H2O
-
 Homo sapiens ?
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Homo sapiens L-aspartate + NH3
-
 ?
3.5.1.1 additional information to become enzymatically active, ASNase3 must undergo autocleavage between residues Gly167 and Thr168 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 heterodimer 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ASNase3
-
 Homo sapiens
3.5.1.1 L-asparaginase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 0.8
-
 beta-L-Asp-L-Phe wild type enzyme, at pH 7.5 and 37°C Homo sapiens
3.5.1.1 3.19
-
 L-asparagine wild type enzyme, at pH 7.5 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 1.52
-
 L-asparagine wild type enzyme, at pH 7.5 and 37°C Homo sapiens
3.5.1.1 6.66
-
 beta-L-Asp-L-Phe wild type enzyme, at pH 7.5 and 37°C Homo sapiens