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Literature summary extracted from

  • Patel, M.; Vasaya, M.M.; Asker, D.; Parsons, R.B.
    HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples: evidence for substrate inhibition kinetics (2013), J. Chromatogr. B, 921-922, 87-95.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.1 imipramine slightly but significantly activates the mouse liver NNMT enzyme and also changes the kinetic parameters Vmax, Km and Ki Mus musculus

Application

EC Number Application Comment Organism
2.1.1.1 medicine inhibition of NNMT activity is a promising therapeutic target for cancer therapy Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.1 nicotinamide the enzyme shows substrate inhibition kinetics Homo sapiens
2.1.1.1 nicotinamide the enzyme shows substrate inhibition kinetics Mus musculus
2.1.1.1 nicotinamide the enzyme shows substrate inhibition kinetics Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.1 additional information
-
 additional information Michaelis-Menten kinetics Mus musculus
2.1.1.1 additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens
2.1.1.1 additional information
-
 additional information Michaelis-Menten kinetics, the Km value cannot be determined using Michaelis-Menten kinetic modelling Oryctolagus cuniculus
2.1.1.1 1.02
-
 nicotinamide pH 7.2, 37°C, liver homogenate Mus musculus
2.1.1.1 1.36
-
 nicotinamide pH 7.2, 37°C, liver homogenate Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.1 cytosol
-
 Mus musculus 5829
-
2.1.1.1 cytosol
-
 Homo sapiens 5829
-
2.1.1.1 cytosol
-
 Oryctolagus cuniculus 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.1 additional information Mus musculus development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview ?
-
 ?
2.1.1.1 additional information Homo sapiens development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview ?
-
 ?
2.1.1.1 additional information Oryctolagus cuniculus development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide Mus musculus
-
 S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide Homo sapiens
-
 S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide Oryctolagus cuniculus
-
 S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.1 Homo sapiens
-
-
-
2.1.1.1 Mus musculus
-
-
-
2.1.1.1 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.1 liver
-
 Mus musculus
-
2.1.1.1 liver
-
 Homo sapiens
-
2.1.1.1 liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.1 additional information development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview Mus musculus ?
-
 ?
2.1.1.1 additional information development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview Homo sapiens ?
-
 ?
2.1.1.1 additional information development and evaluation of a HPLC-UV method for measuring nicotinamide N-methyltransferase activity in biological samples, overview Oryctolagus cuniculus ?
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Mus musculus S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Oryctolagus cuniculus S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.1 NNMT
-
 Mus musculus
2.1.1.1 NNMT
-
 Homo sapiens
2.1.1.1 NNMT
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.1 37
-
 assay at Mus musculus
2.1.1.1 37
-
 assay at Homo sapiens
2.1.1.1 37
-
 assay at Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.1 1.02
-
 nicotinamide pH 7.2, 37°C, liver homogenate Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.1 7.2
-
 assay at Mus musculus
2.1.1.1 7.2
-
 assay at Homo sapiens
2.1.1.1 7.2
-
 assay at Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.1 S-adenosyl-L-methionine
-
 Mus musculus
2.1.1.1 S-adenosyl-L-methionine
-
 Homo sapiens
2.1.1.1 S-adenosyl-L-methionine
-
 Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.1 3.57
-
 nicotinamide pH 7.2, 37°C, liver homogenate Oryctolagus cuniculus
2.1.1.1 20.01
-
 nicotinamide pH 7.2, 37°C, liver homogenate Homo sapiens
2.1.1.1 50.64
-
 nicotinamide pH 7.2, 37°C, liver homogenate Mus musculus