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Literature summary extracted from

  • Wang, C.; Zhan, C.; Cai, Q.; Du, C.; Liu, G.; Su, W.; Cao, M.
    Expression and characterization of common carp (Cyprinus carpio) matrix metalloproteinase-2 and its activity against type I collagen (2014), J. Biotechnol., 177, 45-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.24 gene MMP-2 , cloning from skeletal muscle, DNA and amino acid sequence determination and analysis, sequence comparissons, expression of recombinant His-tagged MMP-2 in truncated form in Escherichia coli inclusion bodies Cyprinus carpio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.24 1,10-phenanthroline complete inhibition Cyprinus carpio
3.4.24.24 EDTA complete inhibition Cyprinus carpio
3.4.24.24 EGTA complete inhibition Cyprinus carpio
3.4.24.24 additional information PMSF, benzamidine, E-64, and pepstatin A are poor inhibitors Cyprinus carpio

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.24 Ba2+ activates Cyprinus carpio
3.4.24.24 Ca2+ divalent metal ion Ca2+ is absolutely necessary for the gelatinolytic activity, suggesting a calcium-dependent metalloproteinase Cyprinus carpio
3.4.24.24 additional information no or poor activation by Zn2+, Mn2+, Mg2+, Cu2+, Fe2+, or Co2+ Cyprinus carpio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.24 Gelatin + H2O Cyprinus carpio
-
 ?
-
 ?
3.4.24.24 Type I collagen + H2O Cyprinus carpio degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.24 Cyprinus carpio M4VLM9 gene MMP-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.24 renaturation of recombinant MMP-2 in truncated form from Escherichia coli strain BL21(DE3) inclusion bodies after nickel affinity chromatography Cyprinus carpio

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.24.24 renaturation of recombinant MMP-2 in truncated form from Escherichia coli strain BL21(DE3) inclusion bodies after nickel affinity chromatography Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.24 skeletal muscle
-
 Cyprinus carpio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.24 Gelatin + H2O
-
 Cyprinus carpio ?
-
 ?
3.4.24.24 Type I collagen + H2O
-
 Cyprinus carpio ?
-
 ?
3.4.24.24 Type I collagen + H2O degradation Cyprinus carpio ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.24 matrix metalloproteinase-2
-
 Cyprinus carpio
3.4.24.24 MMP-2
-
 Cyprinus carpio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.24 40
-
 recombinant enzyme Cyprinus carpio

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.24 30 45 activity range Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.24 8
-
 recombinant enzyme Cyprinus carpio

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.24 7 9 activity range Cyprinus carpio

General Information

EC Number General Information Comment Organism
3.4.24.24 additional information crucial role of the fibronectin-like domain in enzymatic activities, it is involved in substrate binding Cyprinus carpio