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Literature summary extracted from

  • Yang, J.; Li, J.; Mai, Z.; Tian, X.; Zhang, S.
    Purification, characterization, and gene cloning of a cold-adapted thermolysin-like protease from Halobacillus sp. SCSIO 20089 (2013), J. Biosci. Bioeng., 115, 628-632.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.27 gene HSPA, DNA and amino acid sequence determination and analysis, sequence comparison Halobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.27 1,10-phenanthroline 85% inhibition at 1 mM, 93% at 5 mM Halobacillus sp.
3.4.24.27 Cu2+ 39% inhibition at 1 mM, 52% at 5 mM Halobacillus sp.
3.4.24.27 EDTA 77% inhibition at 1 mM, 86% at 5 mM Halobacillus sp.
3.4.24.27 EGTA 59% inhibition at 1 mM, 98% at 5 mM Halobacillus sp.
3.4.24.27 additional information no inhibition by PMSF Halobacillus sp.
3.4.24.27 Ni2+ 39% inhibition at 1 mM, 87% at 5 mM Halobacillus sp.
3.4.24.27 Zn2+ zinc metalloprotease, no effect on activity by exogenous Zn2+ at 1 mM, inhibitory by 69% at 5 mM Halobacillus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.27 extracellular
-
 Halobacillus sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.27 Ba2+ activates 8% at 1 mM, 15% at 5 mM Halobacillus sp.
3.4.24.27 Ca2+ activates 36% at 1 mM, 48% at 5 mM Halobacillus sp.
3.4.24.27 Mg2+ activates 12% at 1 mM, 37% at 5 mM Halobacillus sp.
3.4.24.27 Mn2+ activates 12% at 1 mM, 25% at 5 mM Halobacillus sp.
3.4.24.27 Zn2+ zinc metalloprotease, no efect on activity by exogenous Zn2+ at 1 mM, inhibitory at 5 mM Halobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.27 35000
-
 x * 35000, SDS-PAGE Halobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.27 Halobacillus sp. K0EFJ2 isolated from a marine sediment of South China Sea, gene HSPA
-
3.4.24.27 Halobacillus sp. SCSIO 20089 K0EFJ2 isolated from a marine sediment of South China Sea, gene HSPA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.27 native enzyme to homogeneity from the culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, gel filtration Halobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.27 373
-
 purified native enzyme, pH 8.0, 30°C, substrate feather Halobacillus sp.
3.4.24.27 489
-
 purified native enzyme, pH 8.0, 30°C, substrate elastin Halobacillus sp.
3.4.24.27 697
-
 purified native enzyme, pH 8.0, 30°C, substrate hemoglobin Halobacillus sp.
3.4.24.27 855
-
 purified native enzyme, pH 8.0, 30°C, substrate collagen Halobacillus sp.
3.4.24.27 1468
-
 purified native enzyme, pH 8.0, 30°C, substrate bovine serum albumin Halobacillus sp.
3.4.24.27 2899
-
 purified native enzyme, pH 8.0, 30°C, substrate gelatin Halobacillus sp.
3.4.24.27 3077
-
 purified native enzyme, pH 8.0, 30°C, substrate casein Halobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.27 Bovine serum albumin + H2O
-
 Halobacillus sp. ?
-
 ?
3.4.24.27 Bovine serum albumin + H2O
-
 Halobacillus sp. SCSIO 20089 ?
-
 ?
3.4.24.27 casein + H2O
-
 Halobacillus sp. L-tyrosine + ?
-
 ?
3.4.24.27 Collagen + H2O
-
 Halobacillus sp. ?
-
 ?
3.4.24.27 Collagen + H2O
-
 Halobacillus sp. SCSIO 20089 ?
-
 ?
3.4.24.27 Elastin + H2O
-
 Halobacillus sp. ?
-
 ?
3.4.24.27 feather + H2O
-
 Halobacillus sp. ?
-
 ?
3.4.24.27 Gelatin + H2O
-
 Halobacillus sp. ?
-
 ?
3.4.24.27 Gelatin + H2O
-
 Halobacillus sp. SCSIO 20089 ?
-
 ?
3.4.24.27 Hemoglobin + H2O
-
 Halobacillus sp. ?
-
 ?
3.4.24.27 Hemoglobin + H2O
-
 Halobacillus sp. SCSIO 20089 ?
-
 ?
3.4.24.27 additional information the enzyme has low affinity for alkaline or acid P1' residues, poor activity with N-[3-(2-furyl)acryloyl]-Glu-Glu-OH , no activity with N-[3-(2-furyl)acryloyl]-Ala-Arg-OH Halobacillus sp. ?
-
 ?
3.4.24.27 additional information the enzyme has low affinity for alkaline or acid P1' residues, poor activity with N-[3-(2-furyl)acryloyl]-Glu-Glu-OH , no activity with N-[3-(2-furyl)acryloyl]-Ala-Arg-OH Halobacillus sp. SCSIO 20089 ?
-
 ?
3.4.24.27 N-[3-(2-furyl)acryloyl]-Gly-Phe-NH2 + H2O
-
 Halobacillus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.27 ? x * 35000, SDS-PAGE Halobacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.4.24.27 hspA
-
 Halobacillus sp.
3.4.24.27 thermolysin-like protease
-
 Halobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.27 30
-
-
 Halobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.27
-
-
 cold-adapted thermolysin-like protease, 20% activity remains Halobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.27 8
-
-
 Halobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.27 4 11 active at broad pH intervals Halobacillus sp.