BRENDA - Enzyme Database

The first crystal structure of a dTTP-bound deoxycytidylate deaminase validates and details the allosteric-inhibitor binding site

Marx, A.; Alian, A.; J. Biol. Chem. 290, 682-690 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.5.4.12
expressed in Escherichia coli BL21(DE3) cells
Cyanophage S-TIM5
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.5.4.12
bound to dTTP, hanging drop vapor diffusion method, using 0.2 M potassium formate, 20% (w/v) PEG 3350
Cyanophage S-TIM5
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
N43G
the mutation results in a dramatic loss of activity with less than 20% activity compared to the wild type
Cyanophage S-TIM5
3.5.4.12
T61A
the mutant shows about 65% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
T61D
the mutant shows about 7% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
T61S
the mutant shows about 145% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
T61V
the mutant shows about 50% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
W42A
inactive
Cyanophage S-TIM5
3.5.4.12
W42F
the mutant shows about 118% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
W42Y
the mutant shows about wild type activity
Cyanophage S-TIM5
3.5.4.12
Y116E
inactive
Cyanophage S-TIM5
3.5.4.12
Y116F
the mutant shows about 140% activity compared to the wild type enzyme
Cyanophage S-TIM5
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
-
Cyanophage S-TIM5
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required
Cyanophage S-TIM5
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Cyanophage S-TIM5
-
dUMP + NH3
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.4.12
Cyanophage S-TIM5
H6WFU3
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.4.12
HisPur cobalt resin column chromatography and Superdex 200 gel filtration
Cyanophage S-TIM5
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
734322
Cyanophage S-TIM5
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
no deamination activity with dCTP or CMP
734322
Cyanophage S-TIM5
?
-
-
-
-
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8
-
-
Cyanophage S-TIM5
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
6
8
more than 80% activity between pH 6.0 and 8.0
Cyanophage S-TIM5
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
expressed in Escherichia coli BL21(DE3) cells
Cyanophage S-TIM5
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.5.4.12
bound to dTTP, hanging drop vapor diffusion method, using 0.2 M potassium formate, 20% (w/v) PEG 3350
Cyanophage S-TIM5
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.5.4.12
N43G
the mutation results in a dramatic loss of activity with less than 20% activity compared to the wild type
Cyanophage S-TIM5
3.5.4.12
T61A
the mutant shows about 65% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
T61D
the mutant shows about 7% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
T61S
the mutant shows about 145% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
T61V
the mutant shows about 50% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
W42A
inactive
Cyanophage S-TIM5
3.5.4.12
W42F
the mutant shows about 118% activity compared to the wild type enzyme
Cyanophage S-TIM5
3.5.4.12
W42Y
the mutant shows about wild type activity
Cyanophage S-TIM5
3.5.4.12
Y116E
inactive
Cyanophage S-TIM5
3.5.4.12
Y116F
the mutant shows about 140% activity compared to the wild type enzyme
Cyanophage S-TIM5
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.4.12
dTTP
-
Cyanophage S-TIM5
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.4.12
Mg2+
required
Cyanophage S-TIM5
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.5.4.12
dCMP + H2O
Cyanophage S-TIM5
-
dUMP + NH3
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.4.12
HisPur cobalt resin column chromatography and Superdex 200 gel filtration
Cyanophage S-TIM5
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.4.12
dCMP + H2O
-
734322
Cyanophage S-TIM5
dUMP + NH3
-
-
-
?
3.5.4.12
additional information
no deamination activity with dCTP or CMP
734322
Cyanophage S-TIM5
?
-
-
-
-
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.4.12
8
-
-
Cyanophage S-TIM5
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.4.12
6
8
more than 80% activity between pH 6.0 and 8.0
Cyanophage S-TIM5