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Literature summary extracted from

  • Marx, A.; Alian, A.
    The first crystal structure of a dTTP-bound deoxycytidylate deaminase validates and details the allosteric-inhibitor binding site (2015), J. Biol. Chem., 290, 682-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.12 expressed in Escherichia coli BL21(DE3) cells Cyanophage S-TIM5

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.12 bound to dTTP, hanging drop vapor diffusion method, using 0.2 M potassium formate, 20% (w/v) PEG 3350 Cyanophage S-TIM5

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.12 N43G the mutation results in a dramatic loss of activity with less than 20% activity compared to the wild type Cyanophage S-TIM5
3.5.4.12 T61A the mutant shows about 65% activity compared to the wild type enzyme Cyanophage S-TIM5
3.5.4.12 T61D the mutant shows about 7% activity compared to the wild type enzyme Cyanophage S-TIM5
3.5.4.12 T61S the mutant shows about 145% activity compared to the wild type enzyme Cyanophage S-TIM5
3.5.4.12 T61V the mutant shows about 50% activity compared to the wild type enzyme Cyanophage S-TIM5
3.5.4.12 W42A inactive Cyanophage S-TIM5
3.5.4.12 W42F the mutant shows about 118% activity compared to the wild type enzyme Cyanophage S-TIM5
3.5.4.12 W42Y the mutant shows about wild type activity Cyanophage S-TIM5
3.5.4.12 Y116E inactive Cyanophage S-TIM5
3.5.4.12 Y116F the mutant shows about 140% activity compared to the wild type enzyme Cyanophage S-TIM5

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.12 dTTP
-
 Cyanophage S-TIM5

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.12 Mg2+ required Cyanophage S-TIM5

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.12 dCMP + H2O Cyanophage S-TIM5
-
 dUMP + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.12 Cyanophage S-TIM5 H6WFU3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.12 HisPur cobalt resin column chromatography and Superdex 200 gel filtration Cyanophage S-TIM5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.12 dCMP + H2O
-
 Cyanophage S-TIM5 dUMP + NH3
-
 ?
3.5.4.12 additional information no deamination activity with dCTP or CMP Cyanophage S-TIM5 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.12 DCD
-
 Cyanophage S-TIM5
3.5.4.12 deoxycytidylate deaminase
-
 Cyanophage S-TIM5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.12 8
-
-
 Cyanophage S-TIM5

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.4.12 6 8 more than 80% activity between pH 6.0 and 8.0 Cyanophage S-TIM5