EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.16 | - |
Bos taurus |
1.14.14.16 | expressed in Escherichia coli BL21-Gold DE(3) cells | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.14.16 | in complex with progesterone, sitting drop vapor diffusion method, using 0.1 M sodium HEPES (pH 7.5) containing 0.2 M ammonium sulfate and 25% (w/v) polyethylene glycol 3350 | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.16 | 0.00021 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
1.14.14.16 | 0.00044 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
1.14.14.16 | 0.0005 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
1.14.14.16 | 0.0015 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.14.16 | 50000 | - |
x * 50000, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.16 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | Bos taurus | - |
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.16 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Bos taurus | - |
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.16 | Bos taurus | P00191 | - |
- |
1.14.14.16 | Homo sapiens | P08686 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.16 | - |
Bos taurus |
1.14.14.16 | Ni-NTA agarose column chromatography, and SP Fast-Flow Sepharose column chromatography | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.14.16 | adrenal cortex | - |
Bos taurus | - |
1.14.14.16 | adrenal cortex | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.16 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bos taurus | 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.16 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Bos taurus | 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.16 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.16 | ? | x * 50000, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.16 | CYP21A2 | - |
Bos taurus |
1.14.14.16 | CYP21A2 | - |
Homo sapiens |
1.14.14.16 | cytochrome p450 21A2 | - |
Bos taurus |
1.14.14.16 | cytochrome p450 21A2 | - |
Homo sapiens |
1.14.14.16 | Steroid 21-hydroxylase | - |
Bos taurus |
1.14.14.16 | Steroid 21-hydroxylase | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.16 | 0.078 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
1.14.14.16 | 0.67 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
1.14.14.16 | 2.8 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
1.14.14.16 | 4 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.16 | malfunction | deficiency of this enzyme is involved in about 95% of cases of human congenital adrenal hyperplasia | Bos taurus |
1.14.14.16 | malfunction | deficiency of this enzyme is involved in about 95% of cases of human congenital adrenal hyperplasia | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.16 | 1517 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
1.14.14.16 | 1567 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Bos taurus | |
1.14.14.16 | 2667 | - |
17alpha-hydroxyprogesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
1.14.14.16 | 13330 | - |
progesterone | recombinant enzyme, at pH 7.4 and 37°C | Homo sapiens |