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Literature summary extracted from

  • Pallan, P.S.; Wang, C.; Lei, L.; Yoshimoto, F.K.; Auchus, R.J.; Waterman, M.R.; Guengerich, F.P.; Egli, M.
    Human cytochrome P450 21A2, the major steroid 21-hydroxylase: structure of the enzyme-progesterone substrate complex and rate-limiting C-H bond cleavage (2015), J. Biol. Chem., 290, 13128-13143.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.16
-
 Bos taurus
1.14.14.16 expressed in Escherichia coli BL21-Gold DE(3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.16 in complex with progesterone, sitting drop vapor diffusion method, using 0.1 M sodium HEPES (pH 7.5) containing 0.2 M ammonium sulfate and 25% (w/v) polyethylene glycol 3350 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.16 0.00021
-
 progesterone recombinant enzyme, at pH 7.4 and 37°C Homo sapiens
1.14.14.16 0.00044
-
 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C Bos taurus
1.14.14.16 0.0005
-
 progesterone recombinant enzyme, at pH 7.4 and 37°C Bos taurus
1.14.14.16 0.0015
-
 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.16 50000
-
 x * 50000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.16 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 Bos taurus
-
 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.16 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.16 progesterone + [reduced NADPH-hemoprotein reductase] + O2 Bos taurus
-
 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.16 progesterone + [reduced NADPH-hemoprotein reductase] + O2 Homo sapiens
-
 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.16 Bos taurus P00191
-
-
1.14.14.16 Homo sapiens P08686
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.16
-
 Bos taurus
1.14.14.16 Ni-NTA agarose column chromatography, and SP Fast-Flow Sepharose column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.16 adrenal cortex
-
 Bos taurus
-
1.14.14.16 adrenal cortex
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.16 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Bos taurus 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.16 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.16 progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Bos taurus 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.16 progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.16 ? x * 50000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.14.14.16 CYP21A2
-
 Bos taurus
1.14.14.16 CYP21A2
-
 Homo sapiens
1.14.14.16 cytochrome p450 21A2
-
 Bos taurus
1.14.14.16 cytochrome p450 21A2
-
 Homo sapiens
1.14.14.16 Steroid 21-hydroxylase
-
 Bos taurus
1.14.14.16 Steroid 21-hydroxylase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.16 0.078
-
 progesterone recombinant enzyme, at pH 7.4 and 37°C Bos taurus
1.14.14.16 0.67
-
 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C Bos taurus
1.14.14.16 2.8
-
 progesterone recombinant enzyme, at pH 7.4 and 37°C Homo sapiens
1.14.14.16 4
-
 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.14.16 malfunction deficiency of this enzyme is involved in about 95% of cases of human congenital adrenal hyperplasia Bos taurus
1.14.14.16 malfunction deficiency of this enzyme is involved in about 95% of cases of human congenital adrenal hyperplasia Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.16 1517
-
 progesterone recombinant enzyme, at pH 7.4 and 37°C Bos taurus
1.14.14.16 1567
-
 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C Bos taurus
1.14.14.16 2667
-
 17alpha-hydroxyprogesterone recombinant enzyme, at pH 7.4 and 37°C Homo sapiens
1.14.14.16 13330
-
 progesterone recombinant enzyme, at pH 7.4 and 37°C Homo sapiens