BRENDA - Enzyme Database

Phospholipid flippase activities and substrate specificities of human type IV P-type ATPases localized to the plasma membrane

Takatsu, H.; Tanaka, G.; Segawa, K.; Suzuki, J.; Nagata, S.; Nakayama, K.; Shin, H.W.; J. Biol. Chem. 289, 33543-33556 (2014)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
7.6.2.1
analysis
assay for phospholipid flippase activities of plasma membrane-localized P4-ATPases using human cell lines stably expressing isoforms ATP8B1, ATP8B2, ATP11A, and ATP11C; assay for phospholipid flippase activities of plasma membrane-localized P4-ATPases using human cell lines stably expressing isoforms ATP8B1, ATP8B2, ATP11A, and ATP11C; assay for phospholipid flippase activities of plasma membrane-localized P4-ATPases using human cell lines stably expressing isoforms ATP8B1, ATP8B2, ATP11A, and ATP11C; assay for phospholipid flippase activities of plasma membrane-localized P4-ATPases using human cell lines stably expressing isoforms ATP8B1, ATP8B2, ATP11A, and ATP11C
Homo sapiens
7.6.2.1
medicine
some ATP8B1 mutants found in patients of progressive familial intrahepatic cholestasis type 1 (PFIC1), a severe liver disease caused by impaired bile flow, fail to translocate phosphatidylcholine despite their delivery to the plasma membrane. Incorporation of phosphatidylcholine mediated by ATP8B1 can be reversed by simultaneous expression of ABCB4, a phosphatidylcholine floppase mutated in PFIC3 patients
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.6.2.1
Homo sapiens
O43520
-
-
7.6.2.1
Homo sapiens
P98196
-
-
7.6.2.1
Homo sapiens
P98198
-
-
7.6.2.1
Homo sapiens
Q8NB49
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylcholine/in
-
734287
Homo sapiens
ADP + phosphate + phosphatidylcholine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylcholine/in
preferred substrate
734287
Homo sapiens
ADP + phosphate + phosphatidylcholine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/in
-
734287
Homo sapiens
ADP + phosphate + phosphatidylethanolamine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/in
-
734287
Homo sapiens
ADP + phosphate + phosphatidylserine/out
-
-
-
?
7.6.2.1
additional information
no substrates: phosphatidylcholine, sphingomyelin
734287
Homo sapiens
?
-
-
-
-
Application (protein specific)
EC Number
Application
Commentary
Organism
7.6.2.1
analysis
assay for phospholipid flippase activities of plasma membrane-localized P4-ATPases using human cell lines stably expressing isoforms ATP8B1, ATP8B2, ATP11A, and ATP11C
Homo sapiens
7.6.2.1
medicine
some ATP8B1 mutants found in patients of progressive familial intrahepatic cholestasis type 1 (PFIC1), a severe liver disease caused by impaired bile flow, fail to translocate phosphatidylcholine despite their delivery to the plasma membrane. Incorporation of phosphatidylcholine mediated by ATP8B1 can be reversed by simultaneous expression of ABCB4, a phosphatidylcholine floppase mutated in PFIC3 patients
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.6.2.1
ATP + H2O + phosphatidylcholine/in
-
734287
Homo sapiens
ADP + phosphate + phosphatidylcholine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylcholine/in
preferred substrate
734287
Homo sapiens
ADP + phosphate + phosphatidylcholine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylethanolamine/in
-
734287
Homo sapiens
ADP + phosphate + phosphatidylethanolamine/out
-
-
-
?
7.6.2.1
ATP + H2O + phosphatidylserine/in
-
734287
Homo sapiens
ADP + phosphate + phosphatidylserine/out
-
-
-
?
7.6.2.1
additional information
no substrates: phosphatidylcholine, sphingomyelin
734287
Homo sapiens
?
-
-
-
-
General Information
EC Number
General Information
Commentary
Organism
7.6.2.1
physiological function
ATPase-deficient mutants of isoform ATP11A do not exhibit any flippase activity; ATPase-deficient mutants of isoform ATP11C do not exhibit any flippase activity
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
7.6.2.1
physiological function
ATPase-deficient mutants of isoform ATP11A do not exhibit any flippase activity
Homo sapiens
7.6.2.1
physiological function
ATPase-deficient mutants of isoform ATP11C do not exhibit any flippase activity
Homo sapiens