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Literature summary extracted from

  • Schalk, A.M.; Nguyen, H.A.; Rigouin, C.; Lavie, A.
    Identification and structural analysis of an L-asparaginase enzyme from guinea pig with putative tumor cell killing properties (2014), J. Biol. Chem., 289, 33175-33186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expressed in Escherichia coli BL21(DE3) C41 cells Cavia porcellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.1 sitting drop vapor diffusion method, using 0.1 M HEPES, pH 7.0, 15% (w/v) PEG 4000 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.0577
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Cavia porcellus
-
 L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Cavia porcellus H0W0T5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 Ni-Sepharose column chromatography and Superdex 200 gel filtration Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.1 liver
-
 Cavia porcellus
-
3.5.1.1 serum
-
 Cavia porcellus
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 0°C, 20 mM Tris, pH 7.5, 2 mM dithiothreitol, 1 M KCl, 8 h, the enzyme starts rapidly losing activity Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Cavia porcellus L-aspartate + NH3
-
 ?
3.5.1.1 additional information the enzyme lacks L-glutaminase activity Cavia porcellus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.1 dimer of dimers x-ray crystallography Cavia porcellus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 ASNase1
-
 Cavia porcellus
3.5.1.1 ASPG
-
 Cavia porcellus
3.5.1.1 L-asparaginase
-
 Cavia porcellus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.1 38.6
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.1 800
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus