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Literature summary extracted from

  • Ochi, A.; Makabe, K.; Yamagami, R.; Hirata, A.; Sakaguchi, R.; Hou, Y.M.; Watanabe, K.; Nureki, O.; Kuwajima, K.; Hori, H.
    The catalytic domain of topological knot tRNA methyltransferase (TrmH) discriminates between substrate tRNA and nonsubstrate tRNA via an induced-fit process (2013), J. Biol. Chem., 288, 25562-25574.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.34 expression inb Escherichia coli Escherichia coli
2.1.1.34 expression inb Escherichia coli Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.34 D180A 42% of wild-type activity Thermus thermophilus
2.1.1.34 D190A 5% of wild-type activity Thermus thermophilus
2.1.1.34 E182A 29% of wild-type activity Thermus thermophilus
2.1.1.34 K186A 7% of wild-type activity Thermus thermophilus
2.1.1.34 L179Stop complete loss of activity Thermus thermophilus
2.1.1.34 additional information chimeric proteins of Escherichia coli and Thermus thermophilus enzymes demonstrate that the catalytic domain discriminates substrate tRNAs from nonsubstrate tRNAs. The N- and C-terminal regions do not function in the substrate tRNA discrimination process. The C-terminal region works in the initial binding process, in which nonsubstrate tRNA is not excluded, and structural movement of the motif 2 region of the catalytic domain in an induced-fit process is involved in substrate tRNA discrimination Escherichia coli
2.1.1.34 additional information chimeric proteins of Escherichia coli and Thermus thermophilus enzymes demonstrate that the catalytic domain discriminates substrate tRNAs from nonsubstrate tRNAs. The N- and C-terminal regions do not function in the substrate tRNA discrimination process. The C-terminal region works in the initial binding process, in which nonsubstrate tRNA is not excluded, and structural movement of the motif 2 region of the catalytic domain in an induced-fit process is involved in substrate tRNA discrimination Thermus thermophilus
2.1.1.34 Q185A 16% of wild-type activity Thermus thermophilus
2.1.1.34 V187A 11% of wild-type activity Thermus thermophilus
2.1.1.34 Y184A 10% of wild-type activity Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.34 0.00011
-
guanosine18 in yeast tRNAPhe mutant E182A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.00012
-
guanosine18 in yeast tRNAPhe mutant D180A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.00012
-
guanosine18 in yeast tRNAPhe mutant Q185A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.00014
-
guanosine18 in yeast tRNAPhe mutant V187A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.00015
-
guanosine18 in yeast tRNAPhe wild-type, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.00016
-
guanosine18 in yeast tRNAPhe mutant K186A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.00026
-
guanosine18 in yeast tRNAPhe mutant Y184A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus
2.1.1.34 0.0013
-
guanosine18 in yeast tRNAPhe mutant D190A, pH not specified in the publication, temperature not specified in the publication Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.34 Escherichia coli
-
-
-
2.1.1.34 Thermus thermophilus Q9FAC4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
-
Escherichia coli S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
-
?
2.1.1.34 S-adenosyl-L-methionine + guanosine18 in yeast tRNAPhe
-
Thermus thermophilus S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in yeast tRNAPhe
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.34 tRNA (Gm18) methyltransferase
-
Escherichia coli
2.1.1.34 tRNA (Gm18) methyltransferase
-
Thermus thermophilus