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Literature summary extracted from

  • Rather, L.J.; Weinert, T.; Demmer, U.; Bill, E.; Ismail, W.; Fuchs, G.; Ermler, U.
    Structure and mechanism of the diiron benzoyl-coenzyme A epoxidase BoxB (2011), J. Biol. Chem., 286, 29241-29248.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.208 hanging drop vapor diffusion method, using 1.1 M NaH2PO4 and 0.2 M K2HPO4 as precipitant Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.208 Fe2+ contains two irons per BoxB monomer Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2 Aromatoleum evansii
-
 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.208 Aromatoleum evansii Q9AIX7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.208 Streptavidin affinity column chromatography and gel filtration Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.208 benzoyl-CoA + NADPH + H+ + O2
-
 Aromatoleum evansii 2,3-epoxy-2,3-dihydrobenzoyl-CoA + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.208 benzoyl-coenzyme A epoxidase
-
 Aromatoleum evansii
1.14.13.208 BoxA reductase subunit Aromatoleum evansii
1.14.13.208 BoxB epoxidase subunit Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.208 NADPH
-
 Aromatoleum evansii
1.14.13.208 [4Fe-4S]-center
-
 Aromatoleum evansii