Literature summary extracted from
Rozeboom, H.J.; Bjerkan, T.M.; Kalk, K.H.; Ertesvag, H.; Holtan, S.; Aachmann, F.L.; Valla, S.; Dijkstra, B.W.
Structural and mutational characterization of the catalytic A-module of the mannuronan C-5-epimerase AlgE4 from Azotobacter vinelandii (2008), J. Biol. Chem., 283, 23819-23828.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.1.3.37 |
to 2.1 A resolution. Isoform AlgE4A folds into a right-handed parallel beta-helix structure. The alpha-helix is composed of four parallel beta-sheets, comprising 12 complete turns, and has an amphipathic alpha-helix near the N terminus. The catalytic site is positioned in a positively charged cleft formed by loops extending from the surface encompassing Asp152, an amino acid important for the reaction |
Azotobacter vinelandii |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.1.3.37 |
D173E |
54% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
D178E |
complete loss of activity |
Azotobacter vinelandii |
5.1.3.37 |
D178N |
complete loss of activity |
Azotobacter vinelandii |
5.1.3.37 |
F122Y |
65% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
H154F |
complete loss of activity |
Azotobacter vinelandii |
5.1.3.37 |
H154R |
complete loss of activity |
Azotobacter vinelandii |
5.1.3.37 |
K117 R |
24% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
K117A |
16% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
K255A |
8% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
K255R |
51% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
P153A |
10% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
P153A/D173E |
4% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
Q156A |
10% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
Q225A |
9% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
Q225E |
4% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
Q225N |
6% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
R249A |
46% residual activity |
Azotobacter vinelandii |
5.1.3.37 |
Y149F |
complete loss of activity |
Azotobacter vinelandii |
5.1.3.37 |
Y149H |
complete loss of activity |
Azotobacter vinelandii |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.1.3.37 |
Azotobacter vinelandii |
Q44493 |
- |
- |