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Literature summary extracted from

  • Whittle, E.; Cahoon, E.; Subrahmanyam, S.; Shanklin, J.
    A multifunctional acyl-acyl carrier protein desaturase from Hedera helix L. (English ivy) can synthesize 16- and 18-carbon monoene and diene products (2005), J. Biol. Chem., 280, 28169-28176.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.11 expressed in Escherichia coli BL21 DE3 Gold cells and in Arabidopsis thaliana Hedera helix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.11 0.0015
-
palmitoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix
1.14.19.11 0.0015
-
stearoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix
1.14.19.11 0.002
-
oleoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix
1.14.19.11 0.0021
-
palmitoleoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.11 oleoyl-[acyl-carrier protein] + reduced acceptor + O2 Hedera helix
-
(4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
-
?
1.14.19.11 palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2 Hedera helix
-
(4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
-
?
1.14.19.11 palmitoyl-[acyl-carrier protein] + reduced acceptor + O2 Hedera helix
-
(4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O
-
?
1.14.19.11 stearoyl-[acyl-carrier protein] + reduced acceptor + O2 Hedera helix desaturation occurs at the DELTA9 position ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.11 Hedera helix
-
English ivy
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.11 Poros 20 CM column chromatography and G-300SW gel filtration Hedera helix

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.11 seed
-
Hedera helix
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.11 oleoyl-[acyl-carrier protein] + reduced acceptor + O2
-
Hedera helix (4Z,9Z)-octadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
-
?
1.14.19.11 palmitoleoyl-[acyl-carrier protein] + reduced acceptor + O2
-
Hedera helix (4Z,9Z)-hexadec-4,9-dienoyl-[acyl-carrier protein] + acceptor + H2O
-
?
1.14.19.11 palmitoyl-[acyl-carrier protein] + reduced acceptor + O2
-
Hedera helix (4Z)-hexadec-4-enoyl-[acyl-carrier protein] + acceptor + H2O
-
?
1.14.19.11 stearoyl-[acyl-carrier protein] + reduced acceptor + O2 desaturation occurs at the DELTA9 position Hedera helix ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.11 DELTA4-16:0-ACP desaturase
-
Hedera helix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.11 0.004
-
oleoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix
1.14.19.11 0.048
-
palmitoleoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix
1.14.19.11 0.17
-
stearoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix
1.14.19.11 0.25
-
palmitoyl-[acyl-carrier protein] pH and temperature not specified in the publication Hedera helix