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Literature summary extracted from

  • Wang, Y; Xu, H; Jones, MK; White, R.H.
    Identification of the final two genes functioning in methanofuran biosynthesis in Methanocaldococcus jannaschii (2015), J. Bacteriol., 197, 2850-2858.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.131 expression in Escherichia coli Methanocaldococcus jannaschii
2.7.4.31 expression in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.31 EDTA 10 mM, complete loss of activity Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.31 Mg2+ MfnE exclusively uses Mg2+ as a cofactor to facilitate phosphoryl group transfer Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.131 37000
-
 x * 37000, SDS-PAGE Methanocaldococcus jannaschii
2.7.4.31 24000
-
 x * 24000, SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.131 Methanocaldococcus jannaschii Q58250
-
-
2.5.1.131 Methanocaldococcus jannaschii DSM 2661 Q58250
-
-
2.6.1.108 Methanocaldococcus jannaschii
-
 gene mfnC
-
2.7.4.31 Methanocaldococcus jannaschii Q57900
-
-
2.7.4.31 Methanocaldococcus jannaschii DSM 2661 Q57900
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.31 0.072
-
 substrate AMP, pH 7.0, 80°C Methanocaldococcus jannaschii
2.7.4.31 0.16
-
 substrate 5-(aminomethyl)-3-furanmethanol-phosphate, pH 7.0, 80°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.131 additional information no substrate: tyramine Methanocaldococcus jannaschii ?
-
 ?
2.5.1.131 additional information no substrate: tyramine Methanocaldococcus jannaschii DSM 2661 ?
-
 ?
2.5.1.131 [5-(aminomethyl)furan-3-yl]methyl phosphate + gamma-L-glutamyltyramine
-
 Methanocaldococcus jannaschii (4-[4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl]furan-2-yl)methanamine + diphosphate
-
 ?
2.5.1.131 [5-(aminomethyl)furan-3-yl]methyl phosphate + gamma-L-glutamyltyramine
-
 Methanocaldococcus jannaschii DSM 2661 (4-[4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl]furan-2-yl)methanamine + diphosphate
-
 ?
2.6.1.108 L-alanine + 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate
-
 Methanocaldococcus jannaschii pyruvate + 5-(aminomethyl)-3-furanmethanol-phosphate
-
 ?
2.7.4.31 AMP + ATP
-
 Methanocaldococcus jannaschii 2 ADP
-
 ?
2.7.4.31 AMP + ATP reaction of adenylate kinase, EC 2.7.4.3 Methanocaldococcus jannaschii 2 ADP
-
 ?
2.7.4.31 AMP + ATP
-
 Methanocaldococcus jannaschii DSM 2661 2 ADP
-
 ?
2.7.4.31 AMP + ATP reaction of adenylate kinase, EC 2.7.4.3 Methanocaldococcus jannaschii DSM 2661 2 ADP
-
 ?
2.7.4.31 ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate
-
 Methanocaldococcus jannaschii ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
-
 ?
2.7.4.31 ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate
-
 Methanocaldococcus jannaschii DSM 2661 ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.131 ? x * 37000, SDS-PAGE Methanocaldococcus jannaschii
2.7.4.31 ? x * 24000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.5.1.131 MJ0840
-
 Methanocaldococcus jannaschii
2.6.1.108 mfnC
-
 Methanocaldococcus jannaschii
2.7.4.31 MJ0458
-
 Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.31 7
-
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.5.1.131 physiological function enzyme catalyzes the last step in the biosynthesis of methanofuran core structure , i.e. the condensation of 5-(aminomethyl)-3-furanmethanol-diphosphate with gamma-glutamyltyramine, respectively, to form 4-[N-(gamma-L-glutamyl)-p-(beta-aminoethyl)phenoxymethyl]-(aminomethyl)furan. Condensation of the tyramine moiety with 5-(aminomethyl)-3-furanmethanol-diphosphate occurs after formation of gamma-glutamyltyramine Methanocaldococcus jannaschii
2.6.1.108 evolution all methanofuran structural variants contain a basic core structure of 4-[N-(gamma-L-glutamyl)-p-(bet-aminoethyl)phenoxymethyl]-(aminomethyl)furan (APMF-Glu) but have different side chains depending on the source organism Methanocaldococcus jannaschii
2.6.1.108 metabolism the four genes mfnA, mfnB, mfnC, and mfnD are responsible for the biosynthesis of the methanofuran precursor gamma-glutamyltyramine and 5-(aminomethyl)-3-furanmethanol-phosphate from tyrosine, glutamate, glyceraldehyde-3-phosphate, and alanine in Methanocaldococcus jannaschii, overview Methanocaldococcus jannaschii
2.7.4.31 physiological function enzyme catalyzes the formation of methanofuran precursor 5-(aminomethyl)-3-furanmethanol-diphosphate (F1-PP) from ATP Methanocaldococcus jannaschii