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Literature summary extracted from

  • Franklin, M.J.; Chitnis, C.E.; Gacesa, P.; Sonesson, A.; White, D.C.; Ohman, D.E.
    Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase (1994), J. Bacteriol., 176, 1821-1830.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.1.3.37 analysis in vitro assay for mannuronan C5 epimerization wherein extracts of Escherichia coli expressing high levels of AlgG are incubated with polymannuronate. Epimerization of D-mannuronate to L-guluronate residues in the polymer is detected enzymatically, using a L-guluronate-specific alginate lyase of Klebsieila aerogenes Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.37 expression in Escherichia coli Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.1.3.37 periplasm
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.37 55000
-
 x * 60000, preform, x * 55000, mature protein, SDS-PAGE Pseudomonas aeruginosa
5.1.3.37 60000
-
 x * 60000, preform, x * 55000, mature protein, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.37 Pseudomonas aeruginosa Q51371 isoform AlgG
-
5.1.3.37 Pseudomonas aeruginosa ATCC 15692 Q51371 isoform AlgG
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.1.3.37 proteolytic modification sequence containes a putative N-terminal signal sequence of 35 amino acids Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.37 additional information epimerization reaction is detected only when acetyl groups are removed from the poly-D-mannuronate substrate, suggesting that AlgG epimerization activity in vivo may be sensitive to acetylation of the D-mannuronan residues Pseudomonas aeruginosa ?
-
 ?
5.1.3.37 additional information epimerization reaction is detected only when acetyl groups are removed from the poly-D-mannuronate substrate, suggesting that AlgG epimerization activity in vivo may be sensitive to acetylation of the D-mannuronan residues Pseudomonas aeruginosa ATCC 15692 ?
-
 ?
5.1.3.37 [mannuronan]-beta-D-mannuronate
-
 Pseudomonas aeruginosa [alginate]-alpha-L-guluronate isoform AlgG acts as a polymer-level mannuronan C5-epimerase ?
5.1.3.37 [mannuronan]-beta-D-mannuronate
-
 Pseudomonas aeruginosa ATCC 15692 [alginate]-alpha-L-guluronate isoform AlgG acts as a polymer-level mannuronan C5-epimerase ?

Subunits

EC Number Subunits Comment Organism
5.1.3.37 ? x * 60000, preform, x * 55000, mature protein, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
5.1.3.37 algG
-
 Pseudomonas aeruginosa