BRENDA - Enzyme Database

omega-3 Fatty acid desaturase genes isolated from purslane (Portulaca oleracea L.): Expression in different tissues and response to cold and wound stress

Teixeira, M.; Carvalho, I.; Brodelius, M.; J. Agric. Food Chem. 58, 1870-1877 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.35
gene fad7, DNA and amino acid sequence determination and analysis; gene fad8, DNA and amino acid sequence determination and analysis
Portulaca oleracea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.35
chloroplast membrane
;
Portulaca oleracea
31969
-
1.14.19.35
additional information
ubiquitous expression, tissue-specific levels; ubiquitous expression, tissue-specific levels
Portulaca oleracea
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.35
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Portulaca oleracea
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.35
Portulaca oleracea
A8DTI8
gene fad7
-
1.14.19.35
Portulaca oleracea
C67ZGG8
gene FAD8
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.35
leaf
;
Portulaca oleracea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.35
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734015
Portulaca oleracea
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.35
More
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence; three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.35
Ferredoxin
;
Portulaca oleracea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.35
gene fad7, DNA and amino acid sequence determination and analysis
Portulaca oleracea
1.14.19.35
gene fad8, DNA and amino acid sequence determination and analysis
Portulaca oleracea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.35
Ferredoxin
-
Portulaca oleracea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.35
chloroplast membrane
-
Portulaca oleracea
31969
-
1.14.19.35
additional information
ubiquitous expression, tissue-specific levels
Portulaca oleracea
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.35
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
Portulaca oleracea
-
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.35
leaf
-
Portulaca oleracea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.35
a linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
734015
Portulaca oleracea
an alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.35
More
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
Expression
EC Number
Organism
Commentary
Expression
1.14.19.35
Portulaca oleracea
FAD7 is up-regulated by wounding but not by low temperature. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature; FAD8 is up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature
up
General Information
EC Number
General Information
Commentary
Organism
1.14.19.35
evolution
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence; three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.35
evolution
three histidine clusters characteristic of fatty acid desaturases, a putative chloroplast transit peptide in the N-terminal, and three putative transmembrane domains are identified in the enzyme sequence
Portulaca oleracea
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.19.35
Portulaca oleracea
FAD7 is up-regulated by wounding but not by low temperature. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature
up
1.14.19.35
Portulaca oleracea
FAD8 is up-regulated by cold stress but not by wounding. Total fatty acid and linolenic acid content are higher both, in wounded and intact leaves of plants exposed to low temperature
up