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Literature summary extracted from

  • Hans, M.; Sievers, J.; Müller, U.; Bill, E.; Vorholt, J.A.; Linder, D.; Buckel, W.
    2-Hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum (1999), Eur. J. Biochem., 265, 404-414.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.167 (R)-2-hydroxyglutaryl-CoA dehydratase component A
-
 [Clostridium] symbiosum
4.2.1.167 FMNH2 1 mol per mol heterodimeric enzyme [Clostridium] symbiosum
4.2.1.167 riboflavin 0.3 mol per mol heterodimeric enzyme [Clostridium] symbiosum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.167
-
 [Clostridium] symbiosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.167 4Fe-4S centre the enzyme contains two [4Fe4S]2+ clusters. It is likely that the alpha and beta subunits are bridged by a [4Fe4S]2+ cluster. The enzyme contains 7.5 non-heme iron and 8.1 acid labile sulfur/heterodimer, symmetric cube-type structures [Clostridium] symbiosum
4.2.1.167 [4Fe-4S]2+ cluster 7.3 mol iron per mol heterodimeric enzyme, 8.1 mol sulfur per mol heterodimeric enzyme [Clostridium] symbiosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.167 42000
-
-
 [Clostridium] symbiosum
4.2.1.167 42000
-
 1 * 54000, 1 * 42000, SDS-PAGE [Clostridium] symbiosum
4.2.1.167 54000
-
-
 [Clostridium] symbiosum
4.2.1.167 54000
-
 1 * 54000, 1 * 42000, SDS-PAGE [Clostridium] symbiosum
4.2.1.167 100000
-
 gel filtration [Clostridium] symbiosum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.167 [Clostridium] symbiosum Q9X5B7 and Q9X5B8
-
-
4.2.1.167 [Clostridium] symbiosum Q9X5B8 AND Q9X5B7 Q9X5B8: subunit beta (hgdB), Q9X5B7: subunit alpha (hgdA)
-
4.2.1.167 [Clostridium] symbiosum DSM 934 Q9X5B7 and Q9X5B8
-
-
4.2.1.167 [Clostridium] symbiosum HB 25 Q9X5B8 AND Q9X5B7 Q9X5B8: subunit beta (hgdB), Q9X5B7: subunit alpha (hgdA)
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.167 oxygen sensitive with a half-life of 2 days under aerobic conditions [Clostridium] symbiosum
4.2.1.167 the enzyme is oxygen sensitive with a half-life of 2 days under aerobic conditions [Clostridium] symbiosum
4.2.1.167 under anaerobic conditions at 4°C for about 1 week without loss of activity [Clostridium] symbiosum

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.167
-
 [Clostridium] symbiosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.167 32
-
 strictly anaerobic conditions, substrate (R)-2-hydroxyglutaryl-CoA, 200 mM potassium phosphate pH 7.0, 22°C [Clostridium] symbiosum

Storage Stability

EC Number Storage Stability Organism
4.2.1.167 -80°C, frozen with liquid nitrogen, stable [Clostridium] symbiosum
4.2.1.167 -80°C, liquid nitrogen, long-term storage [Clostridium] symbiosum
4.2.1.167 4°C anaerobic conditions, 1 week stable [Clostridium] symbiosum
4.2.1.167 4°C, 1 week, anaerobic conditions, stable [Clostridium] symbiosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 [Clostridium] symbiosum (E)-glutaconyl-CoA + H2O
-
 r
4.2.1.167 (R)-2-hydroxyglutaryl-CoA
-
 [Clostridium] symbiosum DSM 934 (E)-glutaconyl-CoA + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.167 heterodimer 1 * 54000, 1 * 42000, SDS-PAGE [Clostridium] symbiosum
4.2.1.167 heterodimer 1 * 54000 (alpha) + 1 * 42000 (beta), SDS-PAGE [Clostridium] symbiosum

Synonyms

EC Number Synonyms Comment Organism
4.2.1.167 hgdAB
-
 [Clostridium] symbiosum

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.167 riboflavin 5'-phosphate the enzyme contains 1.0 mol of riboflavin 5'-phosphate per mol of heterodimeric enzyme [Clostridium] symbiosum