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Literature summary extracted from

  • Baig, I.A.; Gedi, V.; Lee, S.C.; Koh, S.H.; Yoon, M.Y.
    Role of a highly conserved proline-126 in ThDP binding of Mycobacterium tuberculosis acetohydroxyacid synthase (2013), Enzyme Microb. Technol., 53, 243-249.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.2.1.6 drug development acetohydroxyacid synthase (AHAS) of Mycobacterium tuberculosis is a promising target for the development of anti-tuberculosis agents Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.6 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis
2.2.1.6 sequence comparisoons, recombinant expression of soluble His-tagged enzyme mutants in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.6 P126A site-directed mutagenesis, the mutant exhibits similar kinetics but significantly lower activity compared to the wild-type enzyme Mycobacterium tuberculosis
2.2.1.6 P126A, the mutant exhibits significantly lower activity than the wild type enzyme Mycobacterium tuberculosis
2.2.1.6 P126E inactive Mycobacterium tuberculosis
2.2.1.6 P126E site-directed mutagenesis, inactive mutant Mycobacterium tuberculosis
2.2.1.6 P126T site-directed mutagenesis, the mutant exhibits significantly lower activity than wild-type enzyme and a significantly decreased preference toward thiamine diphosphate as cofactor Mycobacterium tuberculosis
2.2.1.6 P126T the mutant exhibits significantly lower activity than the wild type enzyme Mycobacterium tuberculosis
2.2.1.6 P126V site-directed mutagenesis, the mutant exhibits significantly lower activity than wild-type enzyme and a significantly decreased preference toward pyruvate as substrate Mycobacterium tuberculosis
2.2.1.6 P126V the mutant exhibits significantly lower activity than the wild type enzyme Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 chlorimuron ethyl a sulfonylurea derivative herbicide Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.6 2.76
-
 pyruvate pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.2.1.6 2.76
-
 pyruvate wild type enzyme, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.2.1.6 6.04
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126A Mycobacterium tuberculosis
2.2.1.6 6.04
-
 pyruvate mutant enzyme P126A, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.2.1.6 466.7
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126V Mycobacterium tuberculosis
2.2.1.6 466.7
-
 pyruvate mutant enzyme P126V, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.2.1.6 807.7
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126T Mycobacterium tuberculosis
2.2.1.6 807.7
-
 pyruvate mutant enzyme P126T, at pH 7.5 and 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.6 Mg2+ required Mycobacterium tuberculosis
2.2.1.6 Mg2+ the enzyme requires a divalent metal ion such as Mg2+ to anchor thiamine diphosphate Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.6 68000
-
 x * 68000, SDS-PAGE Mycobacterium tuberculosis
2.2.1.6 68000
-
 x * 68000, about, wild-type and mutant enzyme variants, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.6 2 pyruvate Mycobacterium tuberculosis
-
 2-acetolactate + CO2
-
 ir
2.2.1.6 2 pyruvate Mycobacterium tuberculosis
-
 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate Mycobacterium tuberculosis H37Rv
-
 2-acetolactate + CO2
-
 ir
2.2.1.6 2 pyruvate Mycobacterium tuberculosis H37Rv
-
 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate Mycobacterium tuberculosis
-
 2-aceto-2-hydroxybutyrate + CO2
-
 ir
2.2.1.6 pyruvate + 2-oxobutyrate Mycobacterium tuberculosis H37Rv
-
 2-aceto-2-hydroxybutyrate + CO2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.6 Mycobacterium tuberculosis
-
-
-
2.2.1.6 Mycobacterium tuberculosis P9WG39
-
-
2.2.1.6 Mycobacterium tuberculosis H37Rv
-
-
-
2.2.1.6 Mycobacterium tuberculosis H37Rv P9WG39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.6
-
 Mycobacterium tuberculosis
2.2.1.6 recombinant soluble His-tagged enzyme mutants from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography, gel filtration, and anion exchange chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ir
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis 2-acetolactate + CO2
-
 ?
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ir
2.2.1.6 2 pyruvate
-
 Mycobacterium tuberculosis H37Rv 2-acetolactate + CO2
-
 ?
2.2.1.6 pyruvate + 2-oxobutyrate
-
 Mycobacterium tuberculosis 2-aceto-2-hydroxybutyrate + CO2
-
 ir
2.2.1.6 pyruvate + 2-oxobutyrate
-
 Mycobacterium tuberculosis H37Rv 2-aceto-2-hydroxybutyrate + CO2
-
 ir

Subunits

EC Number Subunits Comment Organism
2.2.1.6 ? x * 68000, SDS-PAGE Mycobacterium tuberculosis
2.2.1.6 ? x * 68000, about, wild-type and mutant enzyme variants, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.2.1.6 acetohydroxyacid synthase
-
 Mycobacterium tuberculosis
2.2.1.6 AHAS
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.2.1.6 37
-
 assay at Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.6 0.164
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126V Mycobacterium tuberculosis
2.2.1.6 0.164
-
 pyruvate mutant enzyme P126V, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.2.1.6 0.165
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126A Mycobacterium tuberculosis
2.2.1.6 0.165
-
 pyruvate mutant enzyme P126A, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.2.1.6 0.166
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126T Mycobacterium tuberculosis
2.2.1.6 0.166
-
 pyruvate mutant enzyme P126T, at pH 7.5 and 37°C Mycobacterium tuberculosis
2.2.1.6 3.196
-
 pyruvate pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis
2.2.1.6 3.196
-
 pyruvate wild type enzyme, at pH 7.5 and 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.6 7.5
-
 assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.6 FAD dependent on Mycobacterium tuberculosis
2.2.1.6 thiamine diphosphate
-
 Mycobacterium tuberculosis
2.2.1.6 thiamine diphosphate dependent on, the cofactor plays a key role in catalysis. The thiamine diphosphate binding pocket contains the highly conserved proline 126 residue, binding pocket structure, overview. Thiamine diphosphate is located centrally in the active site of AHAS with a unique V-conformation at the dimer interface. In the dimeric structure, one subunit is in contact with the diphosphate moiety of thiamine diphosphate, and the other subunit is in contact with the aminopyrimidine moiety Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.2.1.6 metabolism the enzyme is involved in the branched chain amino acid biosynthesis Mycobacterium tuberculosis
2.2.1.6 additional information structure homology modeling Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.6 0.205
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126T Mycobacterium tuberculosis
2.2.1.6 0.352
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126V Mycobacterium tuberculosis
2.2.1.6 27.22
-
 pyruvate pH 7.5, 37°c, recombinant mutant P126A Mycobacterium tuberculosis
2.2.1.6 1158
-
 pyruvate pH 7.5, 37°C, recombinant wild-type enzyme Mycobacterium tuberculosis