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Literature summary extracted from

  • Thomas, M.G.; Saldanha, M.; Mistry, R.J.; Dexter, D.T.; Ramsden, D.B.; Parsons, R.B.
    Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling (2013), Cell Death Dis., 4, e669.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.1 stable recombinant expression of enzyme NNMT in the SH-SY5Y human neuroblastoma cell line, which has no endogenous enzyme expression, at levels of activity comparable with that in Parkinson's disease brain, recombinant expression of enzyme NNMT in SH-SY5Y human neuroblastoma and N27 rat mesencephalic dopaminergic neurones increases neurite branching, synaptophysin expression and dopamine accumulation and release. Recombinant NNMT-V5 expression in SH-SY5Y cells changes cellular morphology and increases dopamine uptake and release. Transient expression of enzyme NNMT in N27 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.1 additional information enzyme expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.1 S-adenosyl-L-methionine + nicotinamide Homo sapiens
-
 S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.1 additional information no activity in the SH-SY5Y human neuroblastoma cell line and in N27 cells Homo sapiens
-
2.1.1.1 neuron
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.1 S-adenosyl-L-methionine + nicotinamide
-
 Homo sapiens S-adenosyl-L-homocysteine + 1-methylnicotinamide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.1 NNMT
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.1 S-adenosyl-L-methionine
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.1.1 malfunction the enzyme effects on neurons are reduced in cells lacking epinephrin EFNB2 or Akt Homo sapiens
2.1.1.1 metabolism the effects of enzyme NNMT expression in SH-SY5Y cells, e.g. protection against the toxicity of the Complex I (CxI) inhibitors 1-methyl-4-phenylpyridinium ion and rotenone, are mediated via increased CxI activity and ATP production and the sequential activation of the epinephrin EFNB2 and Akt signalling pathways Homo sapiens
2.1.1.1 physiological function the enzyme NNMT expression protects against neurotoxin-mediated cell death by increasing Complex I (CxI) activity, resulting in increased ATP synthesis, mediated via protection of the NDUFS3 subunit of CxI from degradation by increased 1-methylnicotinamide production. The enzyme expression increases neurite branching and the presynaptic marker synaptophysin, synaptophysin expression is induced by the enzyme. 1-Methylnicotinamide replicates the effects of enzyme NNMT expression upon SH-SY5Y neurite branching, effects of NNMT expression on neuron morphology and differentiation, overview. Recombinant NNMT-V5 expression in SH-SY5Y cells changes cellular morphology and increases dopamine uptake and release. The effects of enzyme NNMT expression in SH-SY5Y cells, e.g. protection against the toxicity of the Complex I (CxI) inhibitors 1-methyl-4-phenylpyridinium ion and rotenone, are mediated via increased CxI activity and ATP production and the sequential activation of the epinephrin EFNB2 and Akt signalling pathways. The enzyme NNMT reduces cholinergic phenotype but does not induce terminal differentiation Homo sapiens