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Literature summary extracted from

  • Wang,L.; Li, S.; Yu, W.; Gong, Q.
    Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp. (2015), Biotechnol. Lett., 37, 665-671.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.26 expression in Escherichia coli Shewanella sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.26 Al3+ 1 mM, 56% residual activity Shewanella sp.
4.2.2.26 Ca2+ 1 mM, 80% residual activity Shewanella sp.
4.2.2.26 EDTA 1 mM, 49% residual activity Shewanella sp.
4.2.2.26 Fe3+ 1 mM, 44% residual activity Shewanella sp.
4.2.2.26 Ni2+ 1 mM, 40% residual activity Shewanella sp.
4.2.2.26 SDS 1 mM, 34% residual activity Shewanella sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.26 NaCl 10 mM, 147% of initial activity, 300 mM, 276% of initial activity Shewanella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.26 82000
-
x * 82000, SDS-PAGE Shewanella sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.26 Shewanella sp. A0A088CA41
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.26 proteolytic modification sequence contains a putative signal peptide of 44 amino acid residues Shewanella sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.26 5
-
substrate polyG, pH 7.0, 50°C Shewanella sp.
4.2.2.26 24
-
substrate polyM, pH 7.0, 50°C Shewanella sp.
4.2.2.26 32
-
substrate alginate, pH 7.0, 50°C Shewanella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.26 alginate + H2O
-
Shewanella sp. ? enzyme degrades alginate more efficiently than polyM and polyG block into a monomeric sugar acid ?
4.2.2.26 poly(beta-(1->4)guluronate) + H2O i.e. linear block polymer of alpha-L-guluronic acid Shewanella sp. ?
-
?
4.2.2.26 poly(beta-(1->4)mannuronate) + H2O i.e. linear block polymer of beta-D-mannuronic acid Shewanella sp. ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.26 ? x * 82000, SDS-PAGE Shewanella sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.26 oalS17
-
Shewanella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.26 50
-
-
Shewanella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.26 6.2
-
-
Shewanella sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.26 6 9
-
Shewanella sp.