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Literature summary extracted from

  • Jensen, K.; Johnston, J.B.; de Montellano, P.R.; M?ller, B.L.
    Photosystem I from plants as a bacterial cytochrome P450 surrogate electron donor: terminal hydroxylation of branched hydrocarbon chains (2012), Biotechnol. Lett., 34, 239-245.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.14 0.021
-
 farnesol at pH 7.5 and 25°C Mycobacterium tuberculosis
1.14.15.14 0.036
-
 cholest-4-en-3-one at pH 7.5 and 25°C Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.14 a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ Mycobacterium tuberculosis the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.14 Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.14 a methyl-branched lipid + O2 + reduced ferredoxin [iron-sulfur] cluster + H+ the enzyme uses spinach NAD(P)H-dependent ferredoxin reductase and the [2Fe-2S] iron-sulfur protein ferredoxin as an electron transport system Mycobacterium tuberculosis an omega-hydroxy-methyl-branched lipid + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 cholest-4-en-3-one + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 farnesol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 geranylgeraniol + O2 + reduced ferredoxin [iron-sulfur] cluster + H+
-
 Mycobacterium tuberculosis ? + H2O + oxidized ferredoxin [iron-sulfur] cluster
-
 ?
1.14.15.14 additional information the enzyme shows very low activity towards palmitic acid and 15-methyl-palmitic acid Mycobacterium tuberculosis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.14 CYP124
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.15.14 0.195
-
 cholest-4-en-3-one at pH 7.5 and 25°C Mycobacterium tuberculosis
1.14.15.14 0.258
-
 farnesol at pH 7.5 and 25°C Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.14 NADPH
-
 Mycobacterium tuberculosis