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Literature summary extracted from

  • Meena, B.; Anburajan, L.; Dheenan, P.S.; Begum, M.; Vinithkumar, N.V.; Dharani, G.; Kirubagaran, R.
    Novel glutaminase free L-asparaginase from Nocardiopsis alba NIOT-VKMA08: production, optimization, functional and molecular characterization (2015), Bioprocess Biosyst. Eng., 38, 373-388.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.1 additional information not influenced by Tween-20 Nocardiopsis alba
3.5.1.1 potassium iodide 231.68% activity at 1 mM Nocardiopsis alba
3.5.1.1 Triton X-100 161.26% activity at 1% (v/v) Nocardiopsis alba
3.5.1.1 Tween-80 123.45% activity at 1% (v/v) Nocardiopsis alba

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1 expressed in Escherichia coli JM109 cells Nocardiopsis alba

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 2-mercaptoethanol complete inhibition at 2 mM Nocardiopsis alba
3.5.1.1 Ca2+ 23.69% residual activity at 1 mM Nocardiopsis alba
3.5.1.1 Cd2+ complete inhibition at 1 mM Nocardiopsis alba
3.5.1.1 Co2+ 91.24% residual activity at 1 mM Nocardiopsis alba
3.5.1.1 Cu2+ complete inhibition at 1 mM Nocardiopsis alba
3.5.1.1 cysteine complete inhibition at 1 mM Nocardiopsis alba
3.5.1.1 Hg2+ complete inhibition at 1 mM Nocardiopsis alba
3.5.1.1 iodoacetamide complete inhibition at 1 mM Nocardiopsis alba
3.5.1.1 Mg2+ 29.47% residual activity at 1 mM Nocardiopsis alba
3.5.1.1 Mn2+ 31.56% residual activity at 1 mM Nocardiopsis alba
3.5.1.1 additional information urea and EDTA do not reveal any inhibitory effects on enzyme activity Nocardiopsis alba
3.5.1.1 SDS 33.21% residual activity at 2% (w/v) Nocardiopsis alba
3.5.1.1 Zn2+ 10.43% residual activity at 1 mM Nocardiopsis alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.127
-
 L-asparagine at pH 8.0 and 37°C Nocardiopsis alba

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Fe3+ 145.97% activity at 1 mM Nocardiopsis alba
3.5.1.1 K+ 123.41% activity at 1 mM Nocardiopsis alba
3.5.1.1 Na+ 128.21% activity at 1 mM Nocardiopsis alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.1 L-asparagine + H2O Nocardiopsis alba highly specific substrate L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O Nocardiopsis alba NIOT-VKMA08 highly specific substrate L-aspartate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Nocardiopsis alba W0KM71
-
-
3.5.1.1 Nocardiopsis alba NIOT-VKMA08 W0KM71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1 partially purified by ammonium sulfate precipitation and Q-Sepharose column chromatography Nocardiopsis alba

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.1 1.6
-
 crude enzyme, at pH 8.0 and 37°C Nocardiopsis alba
3.5.1.1 93.28
-
 after 58.44fold purification, at pH 8.0 and 37°C Nocardiopsis alba

Storage Stability

EC Number Storage Stability Organism
3.5.1.1 -20°C, 3 weeks, lyophilized enzyme, no loss of activity Nocardiopsis alba
3.5.1.1 22°C, 24 h, lyophilized enzyme, no loss of activity Nocardiopsis alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O highly specific substrate Nocardiopsis alba L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O highly specific substrate Nocardiopsis alba NIOT-VKMA08 L-aspartate + NH3
-
 ?
3.5.1.1 additional information no activity with L-glutamine, L-aspartic acid, L-glutamic acid, thiourea, L-histidine, glutathione, L-arginine, and glycine Nocardiopsis alba ?
-
 ?
3.5.1.1 additional information no activity with L-glutamine, L-aspartic acid, L-glutamic acid, thiourea, L-histidine, glutathione, L-arginine, and glycine Nocardiopsis alba NIOT-VKMA08 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 AnsA
-
 Nocardiopsis alba
3.5.1.1 L-asparaginase
-
 Nocardiopsis alba
3.5.1.1 L-asparagine amidohydrolase
-
 Nocardiopsis alba

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 37
-
-
 Nocardiopsis alba

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.1 20 75 the enzyme is active between 20 and 75°C. At temperatures above 37°C, reaction rate declines sharply with the loss of 80% of its original activity at 75°C Nocardiopsis alba

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.1 8
-
-
 Nocardiopsis alba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 6.5 10 the enzyme is active between pH 6.5 and 10.0, while at lower pH, enzyme activity is decreasing (20%). There is more than 80% of maximum activity between pH 7.0 and 9.0 Nocardiopsis alba

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.1 8 10 the enzyme preserves more than 90% of its original activity when incubated up to 36 h at pH 8.0-10.0 Nocardiopsis alba