BRENDA - Enzyme Database show

Mutations in the ArsA ATPase that restore interaction with the ArsD metallochaperone

Pillai, J.K.; Venkadesh, S.; Ajees, A.A.; Rosen, B.P.; Bhattacharjee, H.; Biometals 27, 1263-1275 (2014)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
7.3.2.7
ArsD
a metallochaperone
Escherichia coli
Cloned(Commentary)
EC Number
Commentary
Organism
7.3.2.7
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
D137V
inactive
Escherichia coli
7.3.2.7
F120I
inactive
Escherichia coli
7.3.2.7
Q56R
the mutant exhibits significant metalloid-stimulated ATPase activity in vitro
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
As3+
5.5-fold stimulation of ATPase activity at 0.1 mM
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Escherichia coli
-
ADP + phosphate + arsenite/out
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.3.2.7
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
7.3.2.7
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
-
733473
Escherichia coli
ADP + phosphate + arsenite/out
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
7.3.2.7
ArsD
a metallochaperone
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.3.2.7
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
D137V
inactive
Escherichia coli
7.3.2.7
F120I
inactive
Escherichia coli
7.3.2.7
Q56R
the mutant exhibits significant metalloid-stimulated ATPase activity in vitro
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
7.3.2.7
As3+
5.5-fold stimulation of ATPase activity at 0.1 mM
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Escherichia coli
-
ADP + phosphate + arsenite/out
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
7.3.2.7
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
-
733473
Escherichia coli
ADP + phosphate + arsenite/out
-
-
-
?