BRENDA - Enzyme Database show

Mutational analysis of residues in human arsenic (III) methyltransferase (hAS3MT) belonging to 5 A around S-adenosylmethionine (SAM)

Li, X.; Geng, Z.; Chang, J.; Song, X.; Wang, Z.; Biochimie 107 Pt B, 396-405 (2014)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
G134A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
G60A
inactive
Homo sapiens
2.1.1.137
G80A
inactive
Homo sapiens
2.1.1.137
G82A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
I101A
inactive
Homo sapiens
2.1.1.137
L160A
inactive
Homo sapiens
2.1.1.137
L77A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
N155A
inactive
Homo sapiens
2.1.1.137
R57A
the mutant's activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
R83A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
S81A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
T104A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
V157A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
V161A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
Y135A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
Y58A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.0012
-
arsenite
mutant enzyme G134A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0018
-
arsenite
mutant enzyme R83A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0022
-
arsenite
mutant enzyme V157A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0026
-
arsenite
mutant enzyme R57A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0027
-
arsenite
mutant enzyme S81A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0032
-
arsenite
mutant enzyme V161A, at pH 7.0 and 37°C; wild type enzyme, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.007
-
arsenite
mutant enzyme T104A, at pH 7.0 and 37°C; mutant enzyme Y135A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0076
-
arsenite
mutant enzyme Y58A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0087
-
arsenite
mutant enzyme G82A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0096
-
arsenite
mutant enzyme L77A, at pH 7.0 and 37°C
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Homo sapiens
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.137
Homo sapiens
Q9HBK9
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733425
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.1.1.137
G134A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
G60A
inactive
Homo sapiens
2.1.1.137
G80A
inactive
Homo sapiens
2.1.1.137
G82A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
I101A
inactive
Homo sapiens
2.1.1.137
L160A
inactive
Homo sapiens
2.1.1.137
L77A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
N155A
inactive
Homo sapiens
2.1.1.137
R57A
the mutant's activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
R83A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
S81A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
T104A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
V157A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
V161A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
Y135A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
2.1.1.137
Y58A
the mutant’s activity is seriously impaired compared with that of wild type
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.1.1.137
0.0012
-
arsenite
mutant enzyme G134A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0018
-
arsenite
mutant enzyme R83A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0022
-
arsenite
mutant enzyme V157A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0026
-
arsenite
mutant enzyme R57A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0027
-
arsenite
mutant enzyme S81A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0032
-
arsenite
mutant enzyme V161A, at pH 7.0 and 37°C; wild type enzyme, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.007
-
arsenite
mutant enzyme T104A, at pH 7.0 and 37°C; mutant enzyme Y135A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0076
-
arsenite
mutant enzyme Y58A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0087
-
arsenite
mutant enzyme G82A, at pH 7.0 and 37°C
Homo sapiens
2.1.1.137
0.0096
-
arsenite
mutant enzyme L77A, at pH 7.0 and 37°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
Homo sapiens
-
S-adenosyl-L-homocysteine + methylarsonate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.137
S-adenosyl-L-methionine + arsenite
-
733425
Homo sapiens
S-adenosyl-L-homocysteine + methylarsonate
-
-
-
?